Differential responses of antioxidant enzymes to aluminum toxicity in two rice (Oryza sativa L.) cultivars with marked presence and elevated activity of Fe SOD and enhanced activities of Mn SOD and catalase in aluminum tolerant cultivar
Differential responses of antioxidant enzymes to aluminum toxicity in two rice (Oryza sativa L.) cultivars with marked presence and elevated activity of Fe SOD and enhanced activities of Mn SOD and catalase in aluminum tolerant cultivar
复制标题
DOI：
10.1007/s10725-013-9824-5
复制
发表时间：
2013-12-01
影响因子：
4.2
通讯作者：
Dubey, R. S.
中科院分区：
文献类型：
--
作者：
Bhoomika, Kumari;Pyngrope, Samantha;Dubey, R. S.
两种籼稻（Oryza sativa L.）的幼苗。使用不同铝耐受性的HUR-105和Vandana来确定其对铝毒性的不同行为的关键机制。简历。在421亩M Al3+的作用下，hr -105的根冠长、鲜重、干重和含水量显著降低（p < 0.01），表现出对铝的敏感性。Vandana似乎对Al3+相当宽容。在铝敏感品种中，根和茎干质量显著降低。hr -105加178亩Al3+处理3天。在这两个水稻品种中，铝都很容易被根吸收并转运到芽上。吸收铝在根中的定位总是大于在茎中的定位。我们对活性氧（ROS） H2O2和O-2的生成以及主要抗氧化酶如总超氧化物歧化酶（SOD）、Cu/Zn SOD、Mn SOD、Fe SOD、过氧化氢酶（CAT）和愈创木酚过氧化物酶（愈创木酚过氧化物酶）的活性的研究结果表明，铝诱导了cv中更高的氧化应激、更高的ROS生成和更低的ROS清除能力。h -105比Vandana强。在铝处理下，茎部的氧化胁迫高于根。铝处理显著提高了cv幼苗的SOD（尤其是Fe和Mn SOD）和CAT的活性。Vandana提出了这些酶在铝耐受性中的作用。此外，耐铝cv幼苗的根和芽中存在显著的Fe SOD。Vandana在铝处理后活性显著升高（p a千分之0.01），这似乎是该品种的独特特征，表明铁超氧化物歧化酶在水稻耐铝性中的重要作用。
Seedlings of two Indica rice (Oryza sativa L.) cvs. HUR-105 and Vandana, differing in Al-tolerance were used to identify the key mechanisms involved in their differential behaviour towards Al toxicity. Cv. HUR-105 appeared to be Al sensitive by showing significant reduction (p a parts per thousand currency sign 0.01) in root/shoot length, fresh weight, dry weight and water content in presence of 421 mu M Al3+ in growth medium whereas cv. Vandana appeared to be fairly Al3+ tolerant. A conspicuous and significant reduction in dry weight of root and shoot was observed in Al sensitive cv. HUR-105 with 178 mu M Al3+ treatment for 3 days. Al was readily taken up by the roots and transported to shoots in both the rice cultivars. Localization of absorbed Al was always greater in roots than in shoots. Our results of the production of reactive oxygen species (ROS) H2O2 and O-2 (.-) and activities of major antioxidant enzymes such as total superoxide dismutase (SOD), Cu/Zn SOD, Mn SOD, Fe SOD, catalase (CAT) and guaiacol peroxidase revealed Al induced higher oxidative stress, greater production of ROS and lesser capacity to scavenge ROS in cv. HUR-105 than Vandana. With Al treatment, higher oxidative stress was noted in shoots than in roots. Greatly enhanced activities of SOD (especially Fe and Mn SOD) and CAT in Al treated seedlings of cv. Vandana suggest the role of these enzymes in Al tolerance. Furthermore, a marked presence of Fe SOD in roots and shoots of the seedlings of Al tolerant cv. Vandana and its significant (p a parts per thousand currency sign 0.01) increase in activity due to Al-treatment, appears to be the unique feature of this cultivar and indicates a vital role of Fe SOD in Al-tolerance in rice.