Involvement of glutamate release in substance P-induced phase delays of suprachiasmatic neuron activity rhythm in vitro
Involvement of glutamate release in substance P-induced phase delays of suprachiasmatic neuron activity rhythm in vitro
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DOI：
10.1016/s0006-8993(99)01565-6
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发表时间：
1999-07-31
期刊：
影响因子：
2.9
通讯作者：
Watanabe, S
中科院分区：
文献类型：
--
作者：
Hamada, T;Yamanouchi, S;Watanabe, S
视交叉上核（SCN）被认为是哺乳动物的昼夜节律钟。在授时标时间13-14时用P物质（SP）处理离体SCN神经元，使其自发神经活动的昼夜节律出现相位延迟。不仅用SP受体拮抗剂Spantide，而且用N-甲基-D-天冬氨酸受体拮抗剂MK-801处理，SP诱导的相位延迟被阻断。在生化实验中，我们证明了SP诱导的谷氨酸释放从SCN切片观察到的高效液相色谱法。本研究结果表明，谷氨酸释放可能参与SP诱导的相位延迟。(C)1999 Elsevier Science B. V.保留所有权利。
The suprachiasmatic nucleus (SCN) has been identified as a mammalian circadian rhythm clock. Treatment with substance P (SP) at zeitgeber time 13-14 produced phase delays of circadian rhythm in spontaneous neural activity in SCN neurons in vitro. SP-induced phase delays are blocked by treatment with not only SP receptor antagonist, spantide, but N-methyl-D-aspartate receptor antagonist, MK-801. In the biochemical experiment, we demonstrated that SP-induced glutamate release from the SCN slices was observed by the high-performance liquid chromatography assay. The present results suggest that glutamate release may be involved in SP-induced phase delays. (C) 1999 Elsevier Science B.V. All rights reserved.