Trace Enantioselective Determination of Imidazolinone Herbicides in Various Food Matrices Using a Modified QuEChERS Method and Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry
Trace Enantioselective Determination of Imidazolinone Herbicides in Various Food Matrices Using a Modified QuEChERS Method and Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry
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使用改进的 QuEChERS 方法和超高效液相色谱/串联质谱法对各种食品基质中的痕量咪唑啉酮除草剂进行痕量对映选择性测定
DOI：
10.1007/s12161-019-01607-3
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发表时间：
2019-12-01
影响因子：
2.9
通讯作者：
Tan, Huihua
中科院分区：
文献类型：
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作者：
Li, Rui;Hu, Mingfeng;Tan, Huihua
建立了一种改进的QuEChERS方法，用于同时测定两种油料作物（大豆和花生）和三种粮食作物（小麦、玉米和水稻）中3种咪唑啉酮类除草剂（咪草烟、咪草啶酸和咪草烟）的对映体选择性。用20 mL甲醇（用1%乙酸水溶液调节至pH 4）进行样品提取。然后用伯仲胺和石墨化炭黑清洁上清液。对影响该方法性能的几个重要因素进行了优化。在最佳条件下，各对映体的线性关系良好（R2≥ 0.9931），回收率为63.5- 111.5%，相对标准偏差≤ 22.7%。方法的检出限和定量限分别为0.34 ~ 1.5 μg/kg和1.1 ~ 5.0 μg/kg。对于所有三种咪唑啉酮除草剂，S-对映体在R-对映体之前洗脱。最后，几种食品样品（大豆，花生，小麦，玉米，或大米）进行了分析，通过这种方法。三种咪唑啉酮农药（咪草烟、甲氧咪草烟和甲咪唑烟酸）的对映异构体在这些样品中未发现微克/千克水平。
A modified QuEChERS method was developed for simultaneous enantioselective determination of three imidazolinone herbicides (imazethapyr, imazamox, and imazapic) in two oil crops (soybeans and peanuts) and three food crops (wheat, maize, and rice) by chiral reversed-phase ultra-performance liquid chromatography/tandem mass spectrometry. Sample extraction was performed with 20 mL of methanol adjusted to pH 4 with 1% aqueous acetic acid. The supernatant was then cleaned with a primary secondary amine and graphitized carbon black. Several significant factors affecting the performance of the method were optimized. Under the optimum conditions, good linearity (R2≥ 0.9931) and acceptable recoveries (63.5–111.5%, relative standard deviation ≤ 22.7%) were obtained for each enantiomer. The limits of detection and quantification ranged from 0.34 to 1.5 μg/kg and 1.1 to 5.0 μg/kg, respectively. For all three imidazolinone herbicides, theS-enantiomers eluted before theR-enantiomers. Finally, several food samples (soybeans, peanuts, wheat, maize, or rice) were analyzed by this method. The enantiomers of the three imidazolinone pesticides (imazethapyr, imazamox, and imazapic) were not found in these samples at the microgram per kilogram level.