Utility and applications of orthotopic models of human non-small cell lung cancer (NSCLC) for the evaluation of novel and emerging cancer therapeutics.
Utility and applications of orthotopic models of human non-small cell lung cancer (NSCLC) for the evaluation of novel and emerging cancer therapeutics.
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DOI：
10.1002/0471141755.ph1427s62
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发表时间：
2013-10-08
影响因子：
--
通讯作者：
Fields, Alan P
中科院分区：
文献类型：
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作者：
Justilien, Verline;Fields, Alan P
肺癌是全球癌症死亡的主要原因。尽管化疗、放疗和手术取得了进展，但肺癌的5年生存率仍然很低。因此，有必要更好地了解驱动肺肿瘤形成、维持和进展的分子信号传导机制，并将这些知识转化为更好的治疗干预策略。概括晚期人类肺癌临床特征的小鼠模型对于测试新的治疗方法至关重要。本单元描述了一个高度可重复的，易于建立的原位小鼠肺癌模型，提供了体内成像和监测肿瘤生长的方法，并讨论了该模型对转化型肺癌研究和治疗策略发展的有用性。
Lung cancer is a leading cause of cancer deaths worldwide. Despite advances in chemotherapy, radiation therapy, and surgery, lung cancer continues to have a low 5-year survival rate. Thus, there is a need to better understand the molecular signaling mechanisms that drive lung tumor formation, maintenance, and progression, and to translate this knowledge into better therapeutic intervention strategies. Mouse models that recapitulate the clinical features of advanced human lung cancer are critical for testing novel therapeutic approaches. This unit describes a highly reproducible, easy-to-establish orthotopic murine model of lung cancer, provides methods for in vivo imaging and monitoring of tumor growth, and discusses the usefulness of this model for translational lung cancer research and the development of therapeutic strategies.