Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded
Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded
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DOI：
10.7567/jjap.55.03df12
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发表时间：
2016-02
影响因子：
1.5
通讯作者：
K. Tawa;K. Tawa;Chisato Sasakawa;Tsuyoshi Fujita;Tsuyoshi Fujita;K. Kiyosue;C. Hosokawa;J. Nishii;Makoto Oike;Norihiro Kakinuma
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文献类型：
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作者：
K. Tawa;K. Tawa;Chisato Sasakawa;Tsuyoshi Fujita;Tsuyoshi Fujita;K. Kiyosue;C. Hosokawa;J. Nishii;Makoto Oike;Norihiro Kakinuma
在底部面板处具有波长尺度周期性结构的塑料盘被整体模制并涂覆有薄金属膜。被称为等离子体皿的整体模制皿被应用于荧光显微镜下的生物成像。在等离子体基片上，基于光栅耦合表面等离子体共振（GC-SPR）的增强电场可以提供增强的荧光。在这项研究中，两种类型的细胞，人胚肾（HEK）细胞和神经元细胞，在我们的等离子体培养皿中观察。HEK细胞的荧光图像比在常规玻璃底培养皿上获得的荧光图像亮10倍以上。神经元细胞在一体成型的等离子体培养皿上成功培养了10天，在透射光的荧光图像中获得了比落射荧光图像更高的对比度。一体成型的等离子体盘，以及通过UV纳米压印方法制造的等离子体盘，也被发现可用于敏感的生物成像。
A plastic dish with a wavelength-scale periodic structure at a bottom panel was integrally molded and coated with thin metal films. The integrally molded dish called plasmonic dish was applied to bioimaging under a fluorescence microscope. On the plasmonic substrate, the enhanced electric field based on a grating-coupled surface plasmon resonance (GC-SPR) can provide an enhanced fluorescence. In this study, two kinds of cells, human embryonic kidney (HEK) cells and neuronal cells, were observed in our plasmonic dish. Fluorescence images of HEK cells were above 10 times brighter than those obtained on a conventional glass-bottomed dish. Neuronal cells were successfully cultured for 10 d on the plasmonic dish integrally molded, and in fluorescence images with transmitted light, a higher contrast was obtained than in epifluorescence images. The plasmonic dish integrally molded, as well as that fabricated by the UV nanoimprint method, was also found to be useful for sensitive bioimaging.