Identification and characterization of three genes and two pseudogenes on chromosome 13.
Identification and characterization of three genes and two pseudogenes on chromosome 13.
复制标题
13 号染色体上三个基因和两个假基因的鉴定和表征。
DOI：
10.1007/bf02267064
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发表时间：
1996
期刊：
影响因子：
5.3
通讯作者：
Soares,MB
中科院分区：
文献类型：
--
作者：
deFatimaBonaldo,M;Jelenc,P;Su,L;Lawton,L;Yu,MT;Warburton,D;Soares,MB
A study was conducted on the feasibility of isolating genes and pseudogenes that map to chromosome 13 by a hybridization-based approach using a 13-specific library and pools of repeat-free cDNA clones. Five pairs of cDNA and chromosome 13 genomic clones were identified and characterized. Partial or full-length sequence was derived from all cDNAs, and database searches were performed for putative gene identification. Partial sequence was also obtained from the chromosome 13 genomic clones for comparison with those of the hybridizing cDNAs. As a result of these analyses we identified three genes, a putative homologue of a porcine mRNA encoding an unidentified hepatic protein, a putative homologue of a yeast integral membrane protein, and a gene for a translationally controlled tumor protein, and two processed pseudogenes, ribosomal proteins L23a and S3a. The latter was formerly identified as the v-fos transformation effector gene, Fte-1, and recently cited as a possible candidate for the BRCA2 gene on chromosome 13. All genes and pseudogenes were localized to cytogenetic bands by in situ hybridization of metaphase chromosomes with probes derived from the chromosome 13 genomic clones.
