Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.
Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.
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DOI：
10.3390/nu7115440
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发表时间：
2015-10-28
期刊：
影响因子：
5.9
通讯作者：
Verbeke K
中科院分区：
文献类型：
--
作者：
Boets E;Deroover L;Houben E;Vermeulen K;Gomand SV;Delcour JA;Verbeke K
短链脂肪酸（SCFA），包括乙酸盐、丙酸盐和丁酸盐，在人体结肠中未消化的碳水化合物的细菌发酵过程中产生。在这项研究中，我们采用了稳定同位素稀释法来定量在健康人体食用菊粉后，结肠SCFA的体内生产。12名健康受试者进行了一个试验日，在此期间应用[1- 13 C]乙酸盐、[1- 13 C]丙酸盐和[1- 13 C]丁酸盐（分别为12、1.2和0.6 μmol·kg−1·min−1）的预充连续静脉输注。他们在标准早餐中摄入了15 g菊粉。在12小时内，在一天中的定期时间收集呼吸和血液样本。乙酸、丙酸和丁酸的内源性出现率分别为13.3 ± 4.8、0.27 ± 0.09和0.28 ± 0.12 μmol·kg-1·min-1。假设40%、10%和5%的结肠来源的乙酸盐、丙酸盐和丁酸盐进入体循环，估计12小时内结肠菊粉发酵为137 ± 75 mmol乙酸盐、11 ± 9 mmol丙酸盐和20 ± 17 mmol丁酸盐。总之，菊糖主要发酵成乙酸盐，并且在较小程度上发酵成丁酸盐和丙酸盐。稳定同位素技术允许量化体内三种主要SCFA的产生，并被证明是研究SCFA产生的程度和模式的实用工具。
Short chain fatty acids (SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [1-13C]acetate, [1-13C]propionate and [1-13C]butyrate (12, 1.2 and 0.6 μmol·kg−1·min−1, respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 ± 4.8, 0.27 ± 0.09, and 0.28 ± 0.12 μmol·kg−1·min−1, respectively. Colonic inulin fermentation was estimated to be 137 ± 75 mmol acetate, 11 ± 9 mmol propionate, and 20 ± 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.