Initial characterization of an outbreed mouse model for male factor (in)fertility
Initial characterization of an outbreed mouse model for male factor (in)fertility
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DOI：
10.1111/j.2047-2927.2013.00108.x
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发表时间：
2013-09-01
期刊：
影响因子：
4.5
通讯作者：
Weitzel, J. M.
中科院分区：
文献类型：
--
作者：
Michaelis, M.;Langhammer, M.;Weitzel, J. M.
我们分析了一个异交系小鼠，该品系被选择为表型高生育力的158代。在此选择期间，小鼠品系将每窝仔数从10.4增加到17.1，总窝重增加到接近160%。在这项研究中，我们首先描述了高生育力男性的生殖表型。令人惊讶的是，育性系（FL 1）的雄性巴克斯显示出活动和前向活动精子的百分比降低;然而，与对照系相比，其他精子活动性特征（例如速度参数）得到改善。睾丸细胞类型组成的细胞计数调查表明，伴随着减少在睾丸实质中的FL 1的单倍体细胞的二倍体细胞的浓度显着增加。此外，血液中的总睾酮浓度在FL 1中显著增加（> 20 ng/mL）。与睾酮水平的增加一致，我们观察到FL 1睾丸样本中类固醇生成关键酶Cyp 11和Cyp 17的表达率增加。这些数据表明FL 1雄性具有明显的高生育力表型。双等位基因杂交意味着雄性的贡献在很大程度上决定了杂交育种实验中的生殖结果。因此，FL 1是一个有前途的模型，为未来的调查男性因素（在）生育力。我们的观察也可能为人类生殖医学提供有价值的线索。
We analysed an outbreed mouse line which was selected for the phenotype high fertility' for 158 generations. During this selection period the mouse strain increased the number of offspring per litter from 10.4 to 17.1 and the total litter weight up to similar to 160%. In this study, we initially characterize the reproductive phenotype of high fertility males. Surprisingly, male bucks of the fertility line (FL1) show reduced percentage of motile and progressive motile spermatozoa; however, other sperm motility characteristics (e.g. velocity parameters) are improved compared with an unselected control line. Cytometrical investigation of the testicular cell-type composition indicated a significant increased concentration of diploid cells by a concomitant reduction in haploid cells in the testicular parenchyma of FL1. Furthermore, total testosterone concentrations in blood are dramatically increased in FL1 (>20ng/mL). In line with increased testosterone levels, we observed increased expression rates of steroidogenic key enzymes Cyp11 and Cyp17 from FL1 testis samples. These data indicate that FL1 males have a manifest high fertility phenotype'. Diallelic crosses imply that male-only contribution largely determines the reproductive outcome in cross-breeding experiments. FL1 therefore is a promising model for future investigations on male factor (in)fertility. Our observation might also offer valuable cues for human reproductive medicine.