Pt NPs and DNAzyme functionalized polymer nanospheres as triple signal amplification strategy for highly sensitive electrochemical immunosensor of tumour marker
Pt NPs and DNAzyme functionalized polymer nanospheres as triple signal amplification strategy for highly sensitive electrochemical immunosensor of tumour marker
复制标题
铂纳米粒子和DNA酶功能化的聚合物纳米球作为肿瘤标志物高灵敏电化学免疫传感器的三重信号放大策略
DOI：
10.1016/j.bios.2016.06.048
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发表时间：
2016-12-15
影响因子：
12.6
通讯作者：
Guo, Jingang
中科院分区：
文献类型：
--
作者：
Chang, Honghong;Zhang, Haochun;Guo, Jingang
肿瘤标志物的高灵敏度检测是癌症早期诊断的关键。因此，在研制的电化学免疫传感器中，提出了聚合物纳米球、铂纳米粒子和脱氧核酶的三重信号放大策略。首先，通过二茂铁二甲酸的无限配位聚合合成了具有电活性的聚合物纳米球，由于二茂铁分子的丰富，可以产生强烈的电化学信号。此外，利用铂纳米粒子和脱氧核酶(氯化高铁血红素/G-四链体)对聚合物纳米球进行功能化处理，使其具有催化过氧化氢的能力，从而增强了电化学信号。用透射电子显微镜(TEM)和能量色散X-射线能谱(EDX)对产物进行了表征。并用Zeta电位监测了制备过程。在夹心式免疫分析的基础上，构建了以结合物为信号标签的电化学免疫传感器。在最佳条件下，甲胎蛋白的峰面积随甲胎蛋白浓度的增加而增加，线性范围为0.1pgmL(-1)~100ngmL(-1)，检出限为0.086 pgmL(-1)。同时，所设计的免疫传感器具有良好的选择性和抗干扰性、良好的重复性和稳定性。更重要的是，设计的免疫传感器与商品化的酶联免疫吸附试验对实际临床样本的分析没有显著差异。(C)2016爱思唯尔B.V.保留所有权利。
Highly sensitive determination of tumour markers is the key for early diagnosis of cancer. Herein, triple signal amplification strategy resulting from polymer nanospheres, Pt NPs, and DNAzyme was proposed in the developed electrochemical immunosensor. First, electroactive polymer nanospheres were synthesized by infinite coordination polymerization of ferrocenedicarboxylic acid, which could generate strong electrochemical signals due to plentiful ferrocene molecules. Further, the polymer nanospheres were functionalized by Pt NPs and DNAzyme (hemin/G-quadruplex) with the ability of catalyzing H2O2, which contributes to enhance the electrochemical signals. The prepared conjugations were characterized by transmission electron microscope (TEM) and energy dispersive X-ray spectroscopy (EDX). And the process of preparation was monitored by zeta potential. Based on the sandwich-type immunoassay, the electrochemical immunosensor was constructed employing the conjugations as signal tags. Under optimal conditions, the DPV peak increased with the increasing of alpha fetal protein (AFP) concentration, and the linear range was from 0.1 pg mL(-1) to 100 ng mL(-1) with low detection limit of 0.086 pg mL(-1). Meanwhile, the designed immunosensor exhibited excellent selectivity and anti-interference property, good reproducibility and stability. More importantly, there were no significant differences in analyzing real clinical samples between designed immunosensor and commercial ELISA. (C) 2016 Elsevier B.V. All rights reserved.