Insulin-like growth factor-binding protein (IGFBP)-1,-2,-3,-4,-5, and -6 and IGFBP-related protein 1 during rainbow trout postvitellogenesis and oocyte maturation: Molecular characterization, expression profiles, and hormonal regulation
Insulin-like growth factor-binding protein (IGFBP)-1,-2,-3,-4,-5, and -6 and IGFBP-related protein 1 during rainbow trout postvitellogenesis and oocyte maturation: Molecular characterization, expression profiles, and hormonal regulation
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DOI：
10.1210/en.2005-1570
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发表时间：
2006-05-01
期刊：
影响因子：
4.8
通讯作者：
Bobe, J
中科院分区：
文献类型：
--
作者：
Kamangar, BB;Gabillard, JC;Bobe, J
在本研究中，我们报告了虹鳟鱼 IGF 结合蛋白-1 (IGFBP1)、-2、-3、-5 和 -6 和 IGFBP 相关蛋白-1 (IGFBP-rP1) mRNA 的完整编码序列以及 IGFBP-4 mRNA 的部分编码序列。据我们所知，这是 IGFBP4、IGFBP6 和 IGFBPrP1 在非哺乳动物物种中的首次报道。研究了所有 mRNA 的组织分布，并表征了卵黄发生晚期和卵母细胞成熟之间 IGFBP2 至 -6 和 IGFBP-rP1 的卵巢表达谱。此外，还研究了成熟诱导类固醇 17,20 β-二羟基-4-孕-3-酮 (17,20 β P)、促性腺激素和雌二醇对体外激素的调节作用。我们观察到，除了仅在肝脏中发现的IGFBP1之外，IGFBP2至-6和IGFPB-rP1也在排卵前卵巢中表达。 IGFBP3也在肝脏、躯干、肾脏、皮肤和鳃中检测到，而IGFBP2至-6和IGFBP-rP1表现出更广泛的组织分布。在排卵前的卵巢中，IGFBP3在卵黄发生后期间强烈下调，而IGFBP5表现出有限的上调。此外，IGFBP6和IGFBP-rP1在卵母细胞成熟过程中上调。激素调节数据表明，所有卵巢 IGFBP 和 IGFBP-rP1 转录物在促性腺激素刺激下均受到调节，浓度可诱导 100% 卵母细胞成熟。此外，IGFBP2至-5转录本受17,20βP和雌二醇的调节。总之，我们的观察强烈表明，在最终卵母细胞成熟过程中，卵母细胞中 IGFBP3、-4 和 -5 发生下调，以响应促性腺激素和 17,20 β P（IGFBP3 和 -5）刺激，而 IGFBP2 和 -6 上调发生在卵泡层或卵泡外组织中，以响应促性腺刺激。
In the present study we report the full coding sequence of rainbow trout IGF-binding protein-1 (IGFBP1), -2, -3, -5, and -6 and IGFBP-related protein-1 (IGFBP-rP1) mRNAs as well as the partial coding sequence of IGFBP-4 mRNA. To our knowledge, this is the first report of IGFBP4, IGFBP6, and IGFBPrP1 in a nonmammalian species. The tissue distribution of all mRNAs was studied, and the ovarian expression profiles of IGFBP2 to -6 and IGFBP-rP1 between late vitellogenesis and oocyte maturation were characterized. In addition, in vitro hormonal regulation by the maturation-inducing steroid 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta P), gonadotropin, and estradiol were studied. We observed that besides IGFBP1, which was only found in liver, IGFBP2 to -6 and IGFPB-rP1 were expressed in the preovulatory ovary. IGFBP3 was also detected in liver, trunk, kidney, skin, and gills, whereas IGFBP2 to -6 and IGFBP-rP1 exhibited a wider tissue distribution. In the preovulatory ovary, IGFBP3 was strongly downregulated during the postvitellogenesis period, whereas IGFBP5 exhibited a limited up-regulation. In addition, IGFBP6 and IGFBP-rP1 were up-regulated during oocyte maturation. Hormonal regulation data indicated that all ovarian IGFBPs and IGFBP-rP1 transcripts are regulated under gonadotropic stimulation at a concentration that induced 100% oocyte maturation. In addition, IGFBP2 to -5 transcripts are regulated by 17,20 beta P and estradiol. Together, our observations strongly suggest that during final oocyte maturation, a down-regulation of IGFBP3, -4, and -5 occurs in the oocyte in response to gonadotropic and 17,20 beta P (IGFBP3 and -5) stimulation, whereas an up-regulation of IGFBP2 and -6 occurs in follicular layers or extrafollicular tissue in response to gonadotropic stimulation.