Determination of male strobilus developmental stages by cytological and gene expression analyses in Japanese cedar (Cryptomeria japonica)
Determination of male strobilus developmental stages by cytological and gene expression analyses in Japanese cedar (Cryptomeria japonica)
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通过日本雪松（Cryptomeria japonica）的细胞学和基因表达分析确定雄性球果发育阶段
DOI：
10.1093/treephys/tpw001
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发表时间：
2016
期刊：
影响因子：
4
通讯作者：
and Atsushi Watanabe
中科院分区：
文献类型：
--
作者：
Miyoko Tsubomura;Manabu Kurita;and Atsushi Watanabe
控制针叶树中雄性球果发育的分子机制在很大程度上是未知的，因为发育阶段和相关基因尚未被表征。雄性球果发育阶段的确定将有助于针叶树的遗传研究和生殖生物学。我们本研究的目的是通过细胞学和转录组分析确定雄性球果的发育阶段，并确定在 Cryptomeria japonicaD 雄性不育突变体中观察到异常形态的阶段。唐以更好地了解控制雄性球果和花粉发育的分子机制。从萌芽到花粉传播，对雄性球果发育进行了 8 个月的观察。从雄性生殖文库和花粉文库收集的一组 19,209 个表达序列标签 (EST) 用于微阵列分析。我们通过细胞学和转录组分析将雄性球果发育分为10个阶段。八个簇（7324 个 EST）在雄性 strobili 和花粉发育过程中表现出转录组谱的重大变化。粳稻。两个簇分别表现出转录本丰度逐渐增加和下降，而其他六个簇则表现出阶段特异性的变化。利用有关雄性球果和花粉发育阶段以及基因表达谱的信息，鉴定了 Sosyun 雄性不育性状表达的阶段。在第6阶段（小孢子阶段）通过细胞学观察到异常形态，并在第4阶段（四分体阶段）观察到与野生型相比表达模式的差异。
The molecular mechanisms that control male strobilus development in conifers are largely unknown because the developmental stages and related genes have not yet been characterized. The determination of male strobilus developmental stages will contribute to genetic research and reproductive biology in conifers. Our objectives in this study were to determine the developmental stages of male strobili by cytological and transcriptome analysis, and to determine the stages at which aberrant morphology is observed in a male-sterile mutant ofCryptomeria japonicaD. Don to better understand the molecular mechanisms that control male strobilus and pollen development. Male strobilus development was observed for 8 months, from initiation to pollen dispersal. A set of 19,209 expressed sequence tags (ESTs) collected from a male reproductive library and a pollen library was used for microarray analysis. We divided male strobilus development into 10 stages by cytological and transcriptome analysis. Eight clusters (7324 ESTs) exhibited major changes in transcriptome profiles during male strobili and pollen development inC. japonica. Two clusters showed a gradual increase and decline in transcript abundance, respectively, while the other six clusters exhibited stage-specific changes. The stages at which the male sterility trait of Sosyun was expressed were identified using information on male strobilus and pollen developmental stages and gene expression profiles. Aberrant morphology was observed cytologically at Stage 6 (microspore stage), and differences in expression patterns compared with wild type were observed at Stage 4 (tetrad stage).