PROTEIN-STRUCTURE OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS - EVIDENCE FOR A CELL-ASSOCIATED PRECURSOR OF THE VIRION GLYCOPEPTIDES
PROTEIN-STRUCTURE OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS - EVIDENCE FOR A CELL-ASSOCIATED PRECURSOR OF THE VIRION GLYCOPEPTIDES
复制标题
DOI：
10.1016/0042-6822(79)90042-4
复制
发表时间：
1979-01-01
期刊：
影响因子：
3.7
通讯作者：
OLDSTONE, MBA
中科院分区：
文献类型：
--
作者：
BUCHMEIER, MJ;OLDSTONE, MBA
用淋巴细胞性脉络丛脑膜炎病毒（LCMV）感染的细胞[幼仓鼠肾BHK-21/13 S]的免疫沉淀物含有一个74，000 - 75，000道尔顿的糖肽（GP-C），该糖肽在病毒粒子中未发现。用二维肽图和脉冲追踪法研究了GP-C与病毒结构糖肽GP-1和GP-2的关系。GP-C含有GP-1和GP-2的肽，表明它是结构糖肽的蛋白水解切割前体。放射性标记的葡萄糖胺或甲硫氨酸在1小时脉冲期间掺入GP-C中，在6小时的追踪间隔后，在GP-1和GP-2中得到证实。GP-1和GP-2显然是GP-C裂解的唯一主要产物，因为GP-1和GP-2的裂解物的混合物基本上再现了GP-C的图谱。糖标记掺入病毒糖肽中显示GP-C相对富含甘露糖和葡萄糖胺，但岩藻糖和半乳糖贫乏。GP-1含有葡萄糖胺、岩藻糖和半乳糖，而GP-2含有葡萄糖胺和岩藻糖。通过表面碘化和免疫沉淀，只有GP-1被发现在感染的细胞表面。
Immunoprecipitates of cells [baby hamster kidney BHK-21/13S] infected with lymphocytic choriomeningitis virus (LCMV) contain a 74,000-75,000 dalton glycopeptide (GP-C) which is not found in the virion. The relationship between GP-C and the virus structural glycopeptides GP-1 and GP-2 was studied by 2-dimensional peptide mapping and pulse-chase labeling. GP-C contained the peptides of both GP-1 and GP-2 indicating that it is a proteolytic cleavage precursor of the structural glycopeptides. Radiolabeled glucosamine or methionine incorporated into GP-C during a 1 h pulse period was demonstrated in GP-1 and GP-2 after a chase interval of 6 h. GP-1 and GP-2 are apparently the only major products of the cleavages of GP-C, as mixtures of the digests of GP-1 and GP-2 essentially reproduce the map of GP-C. Carbohydrate label incorporation into the viral glycopeptides showed that GP-C was relatively rich in mannose and glucosamine but poor in fucose and galactose. GP-1 contained glucosamine, fucose and galactose, while GP-2 contained glucosamine and fucose. By surface iodination and immunoprecipitation, only GP-1 was found on the surfaces of infected cells.