Nitrative stress through formation of 8-nitroguanosine: Insights into microbial pathogenesis
Nitrative stress through formation of 8-nitroguanosine: Insights into microbial pathogenesis
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DOI：
10.1016/j.niox.2005.10.004
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发表时间：
2006-03-01
影响因子：
3.9
通讯作者：
Akaike, T
中科院分区：
文献类型：
--
作者：
Akuta, T;Zaki, MH;Akaike, T
活性氧和活性氮分别通过各种生物分子（包括蛋白质、脂质和核酸）的氧化和硝化作用介导氧化和硝化应激。我们观察到微生物感染过程中一氧化氮（NO）依赖性的8-硝基鸟苷和3-硝基酪氨酸的形成，我们确定8-硝基鸟苷和3-硝基酪氨酸都是有益的硝化应激生物标志物。然而，重要的是这两种硝化化合物在生物学特性上的巨大差异。8-硝基鸟苷具有3-硝基酪氨酸所不具备的独特的生化和药理学特性，如氧化还原活性和致突变潜能。在这篇综述中，我们讨论了微生物感染过程中发生的硝化应激的机制，特别强调了宿主在对病原体的反应中通过NO形成的8-硝基鸟苷的生物学功能。这些发现为no介导的发病机制提供了新的见解，不仅是病毒感染，还有许多其他疾病。(c) 2005爱思唯尔公司版权所有。
Reactive oxygen and nitrogen species, respectively, mediate oxidative and nitrative stresses by means of oxidation and nitration of varions biomolecules including proteins, lipids, and nucleic acids. We have observed nitric oxide (NO)-dependent formation of 8-nitroguanosine and 3-nitrotyrosine during microbial infection, and we determined that both 8-nitroguanosine and 3-nitrotyrosine are useful biomarkers of nitrative stress. Of importance, however, is the great difference in biological characteristics of these two nitrated compounds. 8-Nitroguanosine has unique biochemical and pharmacological properties such as redox activity and mutagenic potential, which 3-nitrotyrosine does not. In this review, we discuss the mechanism of nitrative stress occurring during microbial infections, with special emphasis on biological functions of 8-nitroguanosine formed via NO during the host response to pathogens. These findings provide insights into NO-mediated pathogenesis not only of viral infections but also of many other diseases. (c) 2005 Elsevier Inc. All rights reserved.