Malignant transformation induced by incorporated radionuclides in BALB/3T3 mouse embryo fibroblasts.
Malignant transformation induced by incorporated radionuclides in BALB/3T3 mouse embryo fibroblasts.
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BALB/3T3 小鼠胚胎成纤维细胞中掺入放射性核素诱导恶性转化。
DOI：
10.1073/pnas.79.24.7763
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发表时间：
1982
影响因子：
11.1
通讯作者：
Little,JB
中科院分区：
文献类型：
--
作者：
LeMotte,PK;Adelstein,SJ;Little,JB
在 BALB/3T3 细胞中体外研究了将 5-[125I]碘脱氧尿苷和[3H]胸苷掺入细胞 DNA 并通过 X 射线照射诱导致死性和恶性转化。在这些条件下，125I辐射高度定位于每次衰变位点的DNA小区域，并高效地产生DNA双链断裂。研究发现，掺入的 125I 每次衰变的致死率是掺入的 3H 的 12-16 倍。然而，对于诱导恶性转化，125I 每次衰变的效率约为 3H 的 25 倍。当比较 125I、3H 和 X 射线在不同生存水平诱导的转化频率时，发现致死率与产生显着细胞杀伤的剂量下的转化密切相关。低剂量时出现例外，125I 在诱导转化方面似乎比 X 射线照射更有效；转化频率等于 125I 暴露所产生的 3-5 Gy X 射线诱导的转化频率，但几乎没有或没有细胞杀伤。
The induction of lethality and malignant transformation by 5-[125I]iododeoxyuridine and [3H]thymidine incorporated into cellular DNA and by x-irradiation was studied in vitro in BALB/3T3 cells. Under these conditions, 125I radiation is highly localized to small regions of the DNA at the site of each decay and produces DNA double-strand breaks with high efficiency. Incorporated 125I was found to be 12-16 times as lethal per decay as incorporated 3H. For the induction of malignant transformation, however, 125I was approximately 25 times as effective per decay as 3H. When the frequencies of transformation induced at various levels of survival by 125I, 3H, and x-rays were compared, lethality was found to correlate closely with transformation at doses that yielded significant cell killing. An exception occurred at low doses, where 125I appeared much more efficient than x-irradiation in inducing transformation; transformation frequencies equal to those induced by 3-5 Gy of x-rays resulted from 125I exposures that yielded little or no cell killing.