STRONG LYMPHOPROLIFERATIVE SUPPRESSIVE FUNCTION OF PLT CLONES SPECIFIC FOR SB-LIKE ANTIGENS
STRONG LYMPHOPROLIFERATIVE SUPPRESSIVE FUNCTION OF PLT CLONES SPECIFIC FOR SB-LIKE ANTIGENS
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DOI：
10.1016/0198-8859(84)90042-9
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发表时间：
1984-01-01
期刊：
影响因子：
2.7
通讯作者：
SCHNEIDER, EM
中科院分区：
文献类型：
--
作者：
PAWELEC, G;WERNET, P;SCHNEIDER, EM
在HLA-A、B和/或Dw/DR匹配但SB不匹配的37种不同的引物组合中，仅从2种抗原中分离出与SB特异性高度一致的T细胞克隆。大多数获得的同种异体增生（PLT）克隆是由与任何目前已知的HLA产物无关的决定因素重新激活的。它们的刺激被单克隆抗体TU39抑制，该抗体优先阻断SB-而不是Dw/ dr相关决定因素的刺激。尽管具有OKT4+， OKT8-， Leu8-表型，并在接触刺激细胞后分泌白细胞介素2，但这些克隆在混合白细胞培养中强烈抑制克隆PLT试剂和未引物淋巴细胞的增殖反应。它们可能代表了一种新型的免疫调节性T细胞，受到sb相关抗原的刺激，尽管它们具有辅助/诱导剂表型，但能够直接抑制淋巴细胞增殖反应。
From a total of 37 different priming combinations between donors matched for HLA-A,B and/or Dw/DR, but mismatched for SB, antigens, T cell clones strongly restimulated with concordance for SB specificities were isolated from only 2. Most of the alloproliferative (PLT) clones obtained were restimulated by determinants not correlated with any currently known HLA product. Their stimulation was inhibited by a monoclonal antibody TU39, which preferentially blocks stimulation by SB-, rather than by Dw/DR-associated determinants. Despite having an OKT4+, OKT8-, Leu8- phenotype and secreting interleukin 2 after contact with stimulatory cells, these clones strongly suppressed proliferative responses of cloned PLT reagents as well as unprimed lymphocytes in mixed leukocyte cultures. They may represent a novel type of immunoregulatory T cell, stimulated by SB-related antigens, which despite their helper/inducer phenotype are able directly to supprss lymphoproliferative responses.