Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters.
Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters.
复制标题
NOS 和小窝蛋白在仓鼠外周脉管系统和骨骼肌中的共同分布。
DOI：
10.1152/ajpheart.1999.277.3.h1167
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Sessa,WC
中科院分区：
文献类型：
--
作者：
Segal,SS;Brett,SE;Sessa,WC
在分离的细胞系统中，一氧化氮合酶(NOS)的活性受小窝蛋白(CAV)的调节，CAV是一种常驻小窝外壳蛋白。由于对活体内这种相互作用知之甚少，我们测试了一氧化氮合酶和小窝蛋白是否一起分布在完整的有机体中。用免疫组织化学方法研究了构成神经元(NNOS)和内皮型一氧化氮合酶(ENOS)亚型以及小窝蛋白-1(CAV-1)和小窝蛋白-3(CAV-3)在金黄地鼠全身血管和外周组织中的分布。观察了颈动脉、腹主动脉、下腔静脉、股动脉和股静脉、颊囊回缩肌的供血动脉和集合静脉、回缩肌和提肌肌的毛细血管和肌纤维，以及颊囊的小动脉和小静脉。在内皮细胞中，eNOS和CAV-1分布于整个血管系统，而nNOS和CAV-3仅存在于毛细血管中，与nNOS反应。在平滑肌细胞中，nNOS和CAV-1也有系统表达，而eNOS不表达；CAV-3存在于动脉血管中，但不存在于静脉血管中。NNOS和CAV-3均位于骨骼肌肌纤维的肌膜上，不含eNOS和CAV-1。这些免疫标记模式提示eNOS和CAV-1在整个内皮细胞中的功能相互作用，血管平滑肌中小窝蛋白亚型对nNOS调控的区域性差异，以及骨骼肌中CAV-3对nNOS的调控。
In isolated cell systems, nitric oxide synthase (NOS) activity is regulated by caveolin (CAV), a resident caveolae coat protein. Because little is known of this interaction in vivo, we tested whether NOS and caveolin are distributed together in the intact organism. Using immunohistochemistry, we investigated the localization of constitutive neuronal (nNOS) and endothelial (eNOS) enzyme isoforms along with caveolin-1 (CAV-1) and caveolin-3 (CAV-3) throughout the systemic vasculature and peripheral tissues of the hamster. The carotid artery, abdominal aorta, vena cava, femoral artery and vein, feed artery and collecting vein of the cheek pouch retractor muscle, capillaries and muscle fibers of retractor and cremaster muscles, and arterioles and venules of the cheek pouch were studied. In endothelial cells, eNOS and CAV-1 were present throughout the vasculature, whereas nNOS and CAV-3 were absent except in capillaries, which reacted for nNOS. In smooth muscle cells, nNOS and CAV-1 were also expressed systemically, whereas eNOS was absent; CAV-3 was present in the arterial but not the venous vasculature. Both nNOS and CAV-3 were located at the sarcolemma of skeletal muscle fibers, which were devoid of eNOS and CAV-1. These immunolabeling patterns suggest functional interactions between eNOS and CAV-1 throughout the endothelium, regional differences in the modulation of nNOS by caveolin isoforms in vascular smooth muscle, and modulation of nNOS by CAV-3 in skeletal muscle.