Lymph node mapping in the mouse
Lymph node mapping in the mouse
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DOI：
10.1016/j.jim.2007.11.012
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发表时间：
2008-03-20
影响因子：
2.2
通讯作者：
Ruddell, Alanna
中科院分区：
文献类型：
--
作者：
Harrell, Maria I.;Iritani, Brian M.;Ruddell, Alanna
小鼠淋巴结的准确识别对于肿瘤转移和免疫后区域免疫反应的研究至关重要。然而，这些小的淋巴器官通常难以用标准的解剖技术在小鼠中识别，因此，较大的大鼠被用来表征啮齿动物的淋巴排水。我们开发了在小鼠脚垫或尾部注射染料的技术，用于标记后腿和腹部、盆腔脏器、前列腺和乳腺的淋巴引流。虽然小鼠和大鼠的淋巴引流模式相似，但腹股沟淋巴结在传入和传出引流方面存在明显差异。这些技术可以准确和快速地识别正常和患病小鼠的淋巴结和淋巴引流。Elsevier B.V.出版
Accurate identification of lymph nodes in the mouse is critical for studies of tumor metastasis, and of regional immune responses following immunization. However, these small lymphatic organs are often difficult to identify in mice using standard dissection techniques, so that larger rats have been used to characterize rodent lymphatic drainage. We developed techniques injecting dye into the mouse footpad or tail, to label the lymphatic drainage of the hind leg and flank, pelvic viscera, prostate and mammary glands. While lymphatic drainage patterns were similar in mice and rats, the inguinal lymph nodes showed distinct differences in afferent and efferent drainage. These techniques allow accurate and rapid identification of lymph nodes and lymphatic drainage in normal as well as diseased mice. Published by Elsevier B.V.