The murine amelogenin promoter: Developmentally regulated expression in transgenic animals
The murine amelogenin promoter: Developmentally regulated expression in transgenic animals
复制标题
DOI：
10.3109/03008209609029173
复制
发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Maxson, RE
中科院分区：
文献类型：
--
作者：
Snead, ML;Paine, ML;Maxson, RE
我们感兴趣的是了解在牙齿发育过程中控制基因表达的等级调节途径。为了寻找成釉细胞在牙齿形成过程中调节淀粉原蛋白表达的分子基础，我们分离了小鼠淀粉原蛋白启动子。对该启动子的分析将为鉴定通过指导性、异种胚层相互作用产生的信号提供更多的细节，这些信号负责淀粉原蛋白基因表达的时间和空间调节。利用转基因小鼠，我们证明了小鼠淀粉原蛋白启动子的2263个核苷酸片段在响应指令信号时，对淀粉原蛋白基因表达进行了适当的时间和空间调控。这些转基因动物是进一步剖析终分化成釉细胞调控基因表达的信号通路的有用试剂。
We are interested in understanding hierarchical regulation pathways that control gene expression in developing teeth. In pursuit of the molecular basis for the regulated expression of amelogenin by developing ameloblasts during tooth formation, we isolated the murine amelogenin promoter, Analysis of this promoter will provide additional details towards the identification of signals generated through instructive-, dissimilar-germ layer interactions that are for responsible for temporal- and spatial-regulation for amelogenin gene expression, Using transgenic mice we demonstrate that a 2263 nucleotide stretch of the murine amelogenin promoter conveys appropriate temporal- and spatial-regulation for amelogenin gene expression in response to instructive-signals. These transgenic animals are useful reagents to further dissect signaling pathways responsible for regulated gene expression by terminally differentiated ameloblasts.