Nitrogen under- and over-supply induces distinct protein responses in maize xylem sap.
Nitrogen under- and over-supply induces distinct protein responses in maize xylem sap.
复制标题
DOI：
10.1111/j.1744-7909.2012.01122.x
复制
发表时间：
2012-06
影响因子：
11.4
通讯作者：
Chengsong Liao;Renyi Liu;Fusuo Zhang;Chunjian Li;Xue-xian Li
中科院分区：
文献类型：
--
作者：
Chengsong Liao;Renyi Liu;Fusuo Zhang;Chunjian Li;Xue-xian Li
在维管植物中，木质部汁液主要将水和矿物质营养物如氮（N）从根运输到枝。然而，它仍然在很大程度上是未知的含氮化合物，特别是木质部汁液中的蛋白质，响应N不足或过度供应。结果表明，减少施氮量可提高玉米全氮中氨基态氮的比例。木质部汁液蛋白质组学分析表明，玉米植株木质部汁液中的23种蛋白质，其中包括12种新鉴定的蛋白质，在不同的氮供应条件下差异积累。这23种蛋白质中有15种主要参与一般的非生物或生物胁迫反应，而其他5种蛋白质似乎主要对N不足或过度供应作出反应，这表明玉米木质部在N不足和过度供应时有不同的蛋白质反应。此外，一个假定的木聚糖酶抑制剂和两个假定的O-糖基水解酶有优先基因表达的芽。
Xylem sap primarily transports water and mineral nutrients such as nitrogen (N) from roots to shoots in vascular plants. However, it remains largely unknown how nitrogenous compounds, especially proteins in xylem sap, respond to N under- or over-supply. We found that reducing N supply increased amino-N percentage of total N in maize (Zea mays L.) xylem sap. Proteomic analysis showed that 23 proteins in the xylem sap of maize plants, including 12 newly identified ones, differentially accumulated in response to various N supplies. Fifteen of these 23 proteins were primarily involved in general abiotic or biotic stress responses, whereas the other five proteins appeared to respond largely to N under- or over-supply, suggesting distinct protein responses in maize xylem upon N under- and over-supply. Furthermore, one putative xylanase inhibitor and two putative O-glycosyl hydrolases had preferential gene expression in shoots.