A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction
A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction
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DOI：
10.1016/j.cplett.2018.11.001
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发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Guo, Chen
中科院分区：
文献类型：
--
作者：
Fan, Qingqian;Guan, Yueping;Guo, Chen
提出了一种通过三步反应制备单分散Fe3O4@SiO2磁性核壳纳米粒子的新方法。用FTIR、SEM和VSM对所得纳米粒子进行了表征，结果表明纳米粒子具有高的饱和磁化强度、良好的表面形貌和分散性。盐酸处理实验结果表明，所得纳米粒子具有优异的耐酸性和稳定致密的硅涂层。进一步测试磁性核壳纳米颗粒用于从大肠杆菌中纯化质粒DNA。coliDH5a细胞。纯化结果表明，与单独的商业提取试剂盒相比，该试剂盒具有更好的吸附、洗脱和纯度结果。
A new method was developed for preparing monodisperse Fe3O4@SiO2 magnetic core-shell nanoparticles via three steps. The obtained nanoparticles exhibit high saturation magnetization, good surface topography and dispersion as characterized by FTIR, SEM and VSM. The experimental results of hydrochloric acid treatment show that the obtained nanoparticles have excellent acid resistance and stable and compact silicon coating. The magnetic core-shell nanoparticles were further tested for purification of plasmid DNA from E. coli DH5a cells. The result of purification leaded to better adsorption, elution and purity results in comparison with isolated commercial extraction kit.