THYMIDINE CATABOLISM BY NORMAL + LEUKEMIC HUMAN LEUKOCYTES
THYMIDINE CATABOLISM BY NORMAL + LEUKEMIC HUMAN LEUKOCYTES
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DOI：
10.1172/jci104911
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发表时间：
1964-01-01
影响因子：
15.9
通讯作者：
PERRY, S
中科院分区：
文献类型：
--
作者：
MARSH, JC;PERRY, S
人白细胞匀浆抑制短期培养的慢性粒细胞白血病细胞对氚标记胸苷的摄取。这种抑制是胸苷磷酸化酶活性的结果，抑制程度与酶活性水平相关。正常白细胞比白血病白细胞含有显著更高水平的胸苷磷酸化酶活性。急性白血病患者的白细胞水平最低。慢性粒细胞白血病缓解期患者的白细胞酶值高于复发患者的白细胞酶值。完整白细胞在磷酸盐缓冲液中孵育后，培养基中也发现胸苷磷酸化酶活性。除了证明胸苷磷酸化酶活性将胸苷转化为胸腺嘧啶外，慢性粒细胞白血病患者的白细胞在体外可将胸腺嘧啶还原为色谱上对应于二氢胸腺嘧啶和/或[β]-脲基异丁酸的化合物。
Human leukocyte homogenates inhibit the uptake of trltiated thymidine by chronic myelocytic leukemic cells in short term culture. This inhibition is the result of thymidine phosphorylase activity and the degree of inhibition correlates with the level of enzyme activity. Normal leukocytes contain significantly higher levels of thymidine phosphorylase activity than leukemic leukocytes. Leukocytes from patients with acute leukemia have the lowest levels. Enzyme values were higher in leukocytes from patients with chronic myelocytic leukemia in remission than in leukocytes from patients in relapse. Thymidine phosphorylase activity is also found in the medium after intact leukocytes are incubated in phosphate buffer. In addition to demonstrating thymidine phosphorylase activity converting thymidine to thy mine, leukocytes of patients with chronic myelocytic leukemia in vitro can reduce thymine to a compound corresponding chromato-graphically to dihydrothymine and/or [beta]-ureidoisobutyric acid.