Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.
Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen.
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吉兰-巴利综合征中的抗外周神经髓磷脂抗体与外周髓磷脂的中性糖脂结合，并与福斯曼抗原发生交叉反应。
DOI：
10.1172/jci114152
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Jungalwala,FB
中科院分区：
文献类型：
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作者：
Koski,CL;Chou,DK;Jungalwala,FB
格林-巴利综合征（GBS）患者急性期血清中含有抗周围神经髓鞘（PNM）的补体结合抗体（Ab）。我们研究了PNM脂质作为这些Ab的推定抗原，因为GBS血清对用蛋白酶处理的PNM保留了显着的反应性。使用C1固定和转移（C1 FT）试验研究了抗体与特定脂质的结合，该试验使用PNM脂质部分重新掺入脂质体作为抗原靶标，或使用过氧化物酶标记的山羊抗人IgM抗体在HPTLC平板上与脂质结合。检测到人PNM的中性糖脂（NGL）与Forssman半抗原（Forss）的糖残基数量相似的反应性。GBS患者的抗NGL Ab滴度（50-220 U/ml）显著高于疾病和正常对照（0-5和0-6 U/ml）。我们研究了这些抗体与Forss的可能的抗原交叉反应性，首先用C1 FT测定和含有Forss的脂质体定量Ab活性。所有12份GBS血清的滴度（54-272 U/ml）均显著高于11份疾病对照（0-22 U/ml）和25份正常对照（0-11 U/ml）。在HPTLC平板上，GBS血清Ab与从狗神经或绵羊红细胞中分离的Forss反应。此外，用含有Forss的脂质体完成了来自8个GBS患者血清的80-100%的抗NGL Ab活性和17-97%的抗PNM Ab活性的吸收，而用对照脂质体则没有。在7例GBS患者中，抗NGL Ab活性仅代表抗PNM Ab活性的一部分。这些结果表明，与Forssman半抗原具有抗原交叉反应性的糖脂可能是GBS中一些抗PNM Ab活性的原因。
During acute-phase illness, serum of patients with Guillain-Barre syndrome (GBS) contain complement-fixing antibodies (Ab) to peripheral nerve myelin (PNM). We investigated PNM lipids as putative antigens for these Ab since GBS serum retained significant reactivity to PNM treated with protease. Ab binding to specific lipids was studied with a C1 fixation and transfer (C1FT) assay using fractions of PNM lipid reincorporated into liposomes as antigen targets or to lipids on HPTLC plates with peroxidase-labeled goat Ab to human IgM. Reactivity was detected to a neutral glycolipid (NGL) of human PNM with a similar number of carbohydrates residues to that of Forssman hapten (Forss). Anti-NGL Ab titers in GBS patients (50-220 U/ml) were significantly elevated over disease and normal controls (0-5 and 0-6 U/ml). We studied possible antigenic cross-reactivity of these Ab with Forss by first quantitating Ab activity with C1FT assay and liposomes containing Forss. All 12 GBS sera tested showed titers (54-272 U/ml) significantly elevated over 11 disease controls (0-22 U/ml) and 25 normal controls (0-11 U/ml). GBS serum Ab reacted with Forss isolated from dog nerve or sheep erythrocytes on HPTLC plates. Further, absorption of 80-100% of anti-NGL Ab activity and 17-97% of anti-PNM Ab activity from eight GBS patient serums was accomplished with liposomes containing Forss but not with control liposomes. In seven GBS patients anti-NGL Ab activity represented only a portion of anti-PNM Ab activity. These results suggest that a glycolipid with antigenic cross-reactivity to Forssman hapten may be responsible for some of the anti-PNM Ab activity in GBS.Images