Multifunctional CD4 T cell responses in patients with active tuberculosis.
Multifunctional CD4 T cell responses in patients with active tuberculosis.
复制标题
活动性结核病患者的多功能 CD4 T 细胞反应
DOI：
10.1038/srep00216
复制
发表时间：
2012
影响因子：
4.6
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Qiu Z;Zhang M;Zhu Y;Zheng F;Lu P;Liu H;Graner MW;Zhou B;Chen X
多功能CD 4 T细胞在人类结核病中的作用尚未明确。在这项研究中，我们发现，与潜伏性结核感染者和健康对照组相比，结核病患者PMA/离子霉素刺激的多功能CD 4 T细胞减少，结核分枝杆菌抗原特异性多功能CD 4 T细胞增加。PMA/ionomycin刺激的IFN-γ+IL-2+TNF-α+ CD 4 T细胞反应在涂阳肺结核患者中较涂阴肺结核患者降低。涂阳肺结核患者IFN-γ+IL-2+TNF-α+ CD 4 T细胞百分率与痰涂片抗酸杆菌分级及高分辨CT评分呈负相关。因此，我们的研究结果反驳了结核分枝杆菌抗原特异性多功能Th 1反应可作为结核病保护性免疫相关因素的观点，提示PMA/离子霉素刺激的IFN-γ+IL-2+TNF-α+ CD 4 T细胞减少可用于活动性结核病的临床诊断。
The roles of multifunctional CD4 T cells in human tuberculosis are not well defined. In this study, we found that patients with tuberculosis had decreased PMA/ionomycin stimulated multifunctional CD4 T cells and increasedMycobacterium tuberculosisantigen-specific multifunctional CD4 T cells, when compared to individuals with latent tuberculosis infection and healthy controls. PMA/ionomycin stimulated IFN-γ+IL-2+TNF-α+CD4 T cell responses were decreased in patients with smear-positive tuberculosis compared to those with smear-negative tuberculosis. The percentage of IFN-γ+IL-2+TNF-α+CD4 T cells in smear positive tuberculosis patients negatively correlated with the grade of sputum smear Acid-Fast Bacilli and high-resolution computed tomography score. Therefore, our findings argue against the notion thatMycobacterium tuberculosisantigen-specific multifunctional Th1 responses in peripheral blood can serve as correlates of protective immunity against tuberculosis; they suggest that the decrease in PMA/ionomycin stimulated IFN-γ+IL-2+TNF-α+CD4 T cells may be applied for clinical diagnosis of active tuberculosis.