ALCAM/CD166 is up-regulated in low-grade prostate cancer and progressively lost in high-grade lesions
ALCAM/CD166 is up-regulated in low-grade prostate cancer and progressively lost in high-grade lesions
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DOI：
10.1002/pros.10161
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发表时间：
2003-01-01
期刊：
影响因子：
2.8
通讯作者：
Rosenthal, A
中科院分区：
文献类型：
--
作者：
Kristiansen, G;Pilarsky, C;Rosenthal, A
背景。活化白细胞粘附分子（Activated leukocyte cell adhesion molecule， ALCAM, CD166）在多种组织中表达，包括恶性黑色素瘤、前列腺癌细胞系、前列腺癌。我们旨在阐明ALCAM在正常和恶性前列腺组织中的表达模式。将54对匹配的微解剖前列腺组织（肿瘤和正常）的RNA杂交到定制的4K基因芯片上。冷冻切片免疫组化证实ALCAM的表达。基于芯片的转录分析显示，22%的前列腺癌病例中ALCAM表达上调。免疫组化显示81%的ALCAM表达升高，19%的ALCAM表达降低。这些表达模式与Gleason肿瘤分级显著相关：ALCAM在大多数低级别肿瘤（Gleason分级1-3）中表达上调，而在高级别肿瘤（Gleason分级4级和5级）中表达下调，尽管ALCAM在两个Gleason分级5级肿瘤中表达上调。ALCAM在前列腺癌中的表达通常受到干扰，这可能表明ALCAM在该疾病的进展中起作用。(C) 2002 Wiley-Liss。公司。
BACKGROUND. Activated leukocyte cell adhesion molecule (ALCAM, CD166) is expressed in various tissues, including malignant melanoma, prostate cancer cell lines,and prostate cancer. We aimed to clarify the expression patterns of ALCAM in normal and malignant prostate tissue.METHODS. RNA from 54 matched pairs of microdissected prostate tissue (tumor and normal) was hybridized to a custom built 4K GeneChip. Frozen section immunohistochemistry was used to confirm the ALCAM expression.RESULTS. Chip-based transcript analysis revealed an up-regulation of ALCAM in prostate cancer in 22%, of cases. Immunohistochemistry showed a focally raised ALCAM expression in 81% and a decreased expression in 19%, of cases. These expression patterns correlated significantly with Gleason tumor grade: ALCAM up-regulation Was found in most low-grade tumors (Gleason grade 1-3), whereas down-regulation occurred preferentially in high-grade tumors (Gleason grade 4 and 5), although up-regulation of ALCAM expression was preserved in two Gleason grade 5 tumors.CONCLUSION. ALCAM expression is commonly disturbed in prostate cancer, which might indicate a role of ALCAM in the progression of this disease. (C) 2002 Wiley-Liss. Inc.