Differential expression of ob mRNA in rat adipose tissues in response to insulin.
Differential expression of ob mRNA in rat adipose tissues in response to insulin.
复制标题
大鼠脂肪组织中 ob mRNA 响应胰岛素的差异表达。
DOI：
10.1006/bbrc.1996.0077
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Dohm,GL
中科院分区：
文献类型：
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作者：
Zheng,D;Jones,JP;Usala,SJ;Dohm,GL
最近克隆的肥胖 (ob) 基因编码脂肪特异性 mRNA 和蛋白质，据信可以调节饱腹感和代谢率，从而控制脂肪储存。在本研究中，我们研究了不同脂肪库中大鼠 ob mRNA 水平以及胰岛素输注对 ob mRNA 表达的影响。 Northern blot分析显示腹部脂肪（附睾和肾周脂肪垫）的ob mRNA水平高于皮下脂肪。禁食大鼠注射胰岛素 2.5 小时后，附睾和肾周脂肪垫中的 ob mRNA 水平分别增加了 3.0 倍和 1.9 倍。然而，在皮下脂肪库中没有观察到ob mRNA水平的变化。这些数据证明了 ob mRNA 的脂肪库位点特异性表达，并支持胰岛素在 ob mRNA 表达中发挥重要作用的假设。
The recently cloned obese (ob) gene encodes a fat-specific mRNA and a protein which is believed to regulate satiety and metabolic rate so as to control fat storage. In the present study we investigated rat ob mRNA levels in different fat depots and the effects of insulin infusion on ob mRNA expression. Northern blot analysis showed the abdominal fat (epididymal and perirenal fat pads) had higher ob mRNA levels than subcutaneous fat. After a 2.5-hr infusion of insulin into fasted rats, ob mRNA levels were increased by 3.0 and 1.9-fold in epididymal and perirenal fat pads, respectively. However, no change of ob mRNA level was observed in the subcutaneous fat depot. These data demonstrate adipose depot site-specific expression of ob mRNA and support the hypothesis that insulin plays an important role in the expression of ob mRNA.