Correction for differences in volatility among olfactory stimuli and effect on EAG responses of Dioryctria abietivorella to plant volatiles
Correction for differences in volatility among olfactory stimuli and effect on EAG responses of Dioryctria abietivorella to plant volatiles
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DOI：
10.1023/a:1020930910416
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发表时间：
1999-06-01
影响因子：
2.3
通讯作者：
Grant, GG
中科院分区：
文献类型：
--
作者：
Brockerhoff, EG;Grant, GG
我们描述了一种方法，使用石蜡油溶液和气相色谱法来测量和校正应用于滤纸的测试化合物之间的挥发性差异，并解决溶剂对EAG响应的贡献最小化的问题。为了研究波动性偏差的影响，我们评估了雌松毛虫，Dioryctria abietivorella，五种挥发性植物化合物的EAG反应，使用一种新的方法来规范EAG反应，占随着时间的推移发生的触角敏感性的损失。刺激产生的等摩尔（未校正）的解决方案，或从校正的解决方案，调整产生等摩尔的空气中的浓度在空气中喷出的天线。当测试未校正的溶液时，两种最挥发性的化合物（E）-2-己烯醛和（E）-3-己烯基乙酸酯引起比三种萜烯显著更大的EAG响应。当测试校正浓度时，这些响应的排序改变：（E）-2-己烯醛引起的EAG显著小于（-）-α-蒎烯、（-）-柠檬烯和（E）-3-己烯乙酸酯。在另一方面，有没有效果的EAG反应的排名两个单萜和倍半萜，（-）-反式-香紫苏烯，相对于彼此。EAG数据的标准化不影响总体结果（即，刺激排名），但确实减少了它们在制剂中的差异。结果表明，当化合物具有广泛的不同的挥发性进行比较，在嗅觉生物测定，测试溶液的浓度应进行调整，以产生等摩尔的空气中浓度的排放。
We describe a method using paraffin oil solutions and gas chromatography to measure and correct for differences in volatility among test compounds applied to filter paper and to address the problem of minimizing solvent contributions to EAG responses. To examine the effect of the volatility bias, we evaluated the EAG responses of female fir coneworm, Dioryctria abietivorella, to five volatile plant compounds, using a new method to normalize EAG responses to account for the loss of antennal sensitivity that occurs over time. Stimuli were generated either from equimolar (uncorrected) solutions or from corrected solutions that were adjusted to yield equimolar airborne concentrations in the air puffed over antennae. When uncorrected solutions were tested, the two most volatile compounds, (E)-2-hexenal and (E)-3-hexenyl acetate, elicited significantly larger EAG responses than three terpenes. When corrected concentrations were tested, the ranking of these responses changed: (E)-2-hexenal elicited significantly smaller EAGs than (-)-alpha-pinene, (-)-limonene, and (E)-3-hexenyl acetate. On the other hand, there was no effect on the ranking of EAG responses to the two monoterpenes and a sesquiterpene, (-)-trans-caryophyllene, relative to each other. Normalization of EAG data did not affect the overall results (i.e., stimulus rankings) but did reduce their variance within preparations. The results show that when compounds with widely different volatilities are compared in olfactory bioassays, the concentrations of test solutions should be adjusted to produce emissions with equimolar airborne concentrations.