Expression patterns of sterol transporters NPC1 and NPC2 in the cnidarian-dinoflagellate symbiosis
Expression patterns of sterol transporters NPC1 and NPC2 in the cnidarian-dinoflagellate symbiosis
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DOI：
10.1111/cmi.12753
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发表时间：
2017-10-01
影响因子：
3.4
通讯作者：
Sabourault, Cecile
中科院分区：
文献类型：
--
作者：
Dani, Vincent;Priouzeau, Fabrice;Sabourault, Cecile
线虫(如珊瑚和海葵)和共生藻属的光合甲藻之间的共生相互作用是由寄主-共生体识别过程和两个伙伴之间的代谢交换触发的。无论是在正常条件下，还是在进一步导致共生崩溃的胁迫期间，分子通讯对于共生的动态平衡调节都是至关重要的。因此，重要的是要确定和充分描述共生界面上这种亲密互动的关键参与者。在本研究中，我们研究了NPC1和NPC2在共生海葵Aemonia viridis和Aiptasia sp.中的细胞和亚细胞定位和表达。我们首次证实NPC1定位于宿主组织中的小泡和几个珊瑚虫物种的共生体膜上。我们证明了NPC2-a蛋白主要在表皮中表达，而NPC2-d蛋白与共生体膜密切相关。此外，我们还发现，在健康的共生标本中，NPC2-d蛋白的表达与共生菌的存在有关。由于npc2-d是珊瑚虫特有的复制基因，我们推测它可能起源于一个亚功能化过程，该过程可能导致珊瑚虫获得功能和共生适应。Niemann-Pick C型蛋白可能是功能性共生中的关键角色，是研究珊瑚虫-甲藻联合中宿主-共生体相互作用的有用工具。
The symbiotic interaction between cnidarians (e.g., corals and sea anemones) and photosynthetic dinoflagellates of the genus Symbiodinium is triggered by both host-symbiont recognition processes and metabolic exchange between the 2 partners. The molecular communication is crucial for homeostatic regulation of the symbiosis, both under normal conditions and during stresses that further lead to symbiosis collapse. It is therefore important to identify and fully characterise the key players of this intimate interaction at the symbiotic interface. In this study, we determined the cellular and subcellular localization and expression of the sterol-trafficking Niemann-Pick type C proteins (NPC1 and NPC2) in the symbiotic sea anemones Anemonia viridis and Aiptasia sp. We first established that NPC1 is localised within vesicles in host tissues and to the symbiosome membranes in several anthozoan species. We demonstrated that the canonical NPC2-a protein is mainly expressed in the epidermis, whereas the NPC2-d protein is closely associated with symbiosome membranes. Furthermore, we showed that the expression of the NPC2-d protein is correlated with symbiont presence in healthy symbiotic specimens. As npc2-d is a cnidarian-specific duplicated gene, we hypothesised that it probably arose from a subfunctionalisation process that might result in a gain of function and symbiosis adaptation in anthozoans. Niemann-Pick type C proteins may be key players in a functional symbiosis and be useful tools to study host-symbiont interactions in the anthozoan-dinoflagellate association.