Optical separation of cells on potential energy landscapes: Enhancement with dielectric tagging
Optical separation of cells on potential energy landscapes: Enhancement with dielectric tagging
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DOI：
10.1109/jstqe.2007.911314
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发表时间：
2007-11-01
影响因子：
4.9
通讯作者：
Riches, Andrew C.
中科院分区：
文献类型：
--
作者：
Dholakia, Kishan;Lee, Woei Ming;Riches, Andrew C.
我们回顾了新兴的技术，利用光学力的胶体和细胞样品的微流体分选。我们区分我们称之为被动和主动形式的颗粒分选，其中我们可以使用荧光标记（主动）或仅基于物理属性（被动）进行分选。然后，我们研究细胞分选与光学潜在的景观，如贝塞尔光束和多光束干涉图案。对于这两种形式的光学势能景观，我们进一步提出了通过将介电微球标记到细胞上来增强光学分选过程的可能性。结果表明，标记的方法可以增强细胞的分选，因为它们随后对所施加的光场或势能景观做出更强烈的响应。该技术提供了一种简单的方法来增强排序过程。
We review the emergent techniques of microfluidic sorting of colloidal and cellular samples using optical forces. We distinguish between what we term as passive and active forms of particle sorting where we can sort either with the use of a fluorescent marker (active) or based on physical attributes alone (passive). We then examine cell sorting with optical potential landscapes such as a Bessel light beam and a multibeam interference pattern. For both forms of optical potential energy landscape, we further present the possibility of enhancing the optical sorting process by tagging dielectric microspheres onto the cells. The results suggest that the methodology of tagging can enhance the sorting of cells as they subsequently respond more strongly to an applied optical field or potential energy landscape. This technique presents a simple method to enhance the sorting process.