Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 phases
Comparison of early development in utero of cloned fetuses derived from bovine fetal fibroblasts at the G1 and G0/G1 phases
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DOI：
10.1016/j.anireprosci.2010.02.006
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Saeki, Kazuhiro
中科院分区：
文献类型：
--
作者：
Ideta, Atsushi;Hayama, Koh;Saeki, Kazuhiro
在牛体细胞核移植（NT）中，当胚胎来源于C1期的成纤维细胞而不是G 0/G1期的细胞时，它们更有可能发育至足月。为了更好地理解这种差异的原因，我们使用C1期细胞（G1-NT胚胎）和G 0/G1期细胞（G 0/G1-NT胚胎）检查了NT胚胎妊娠早期的形态发育。将来自G1和G 0/G1-NT胚胎的囊胚转移到受体小母牛中，并使用前列腺素F-2 α和催产素非手术地取回妊娠第50天的孕体。以体外受精（IVF）、孤雌生殖和人工授精（AI）胚胎作为对照。C1和G 0/G1-NT胚胎发育到囊胚期的胚胎百分比没有差异。携带G1-NT、G 0/G1-NT、IVF、孤雌生殖和AI胚胎的受体小母牛在第30天的妊娠率相似（57-100%）。两个受体小母牛携带孤雌生殖胚胎返回到发情期之间的30和50天的妊娠，而所有其他妊娠仍然可行。所有实验组中妊娠第50天的大多数胎儿（83%）通过几种PGF（2 α）和催产素治疗非手术恢复。G1-NT胚胎、IVF胚胎和AI胚胎的正常胚胎复苏率分别为83%、80%和88%，高于G 0/G1-NT胚胎的复苏率（33%）和孤雌胚的复苏率（0%）。我们的研究结果表明，NT胚胎重建与细胞在Cl期有较高的发育能力，从胚胎移植到妊娠50天。(C)2010爱思唯尔有限公司版权所有。
In bovine somatic cell nuclear transfer (NT), embryos are more likely to develop to full term when they are derived from fibroblasts at the Cl phase instead of cells at the G0/G1 phase. To better understand the reason for this difference, we examined morphological development in the early pregnancy of NT embryos using Cl phase cells (G1-NT embryos) and G0/G1 phase cells (G0/G1-NT embryos). Blastocysts derived from G1 and G0/G1-NT embryos were transferred to recipient heifers, and the conceptuses at day 50 of gestation were retrieved non-surgically using prostaglandin F-2 alpha and oxytocin. In vitro-fertilized (IVF), parthenogenetic and artificially inseminated (AI) embryos were used as controls. The percentages of embryos that developed to the blastocyst stage did not differ between Cl and G0/G1-NT embryos. Pregnancy rates at day 30 of recipient heifers carrying G1-NT, G0/G1-NT, IVF, parthenogenetic and AI embryos were similar (57-100%). Two recipient heifers carrying parthenogenetic embryos returned to estrus between days 30 and 50 of gestation, whereas all other pregnancies remained viable. Most fetuses at day 50 of gestation of all experimental groups (83%) were recovered non-surgically by several PGF(2 alpha) and oxytocin treatments. Recovery rates of normal fetuses derived from G1-NT embryos (83%), IVF embryos (80%) and AI embryos (88%) were greater than those of G0/G1-NT embryos (33%) and parthenogenetic embryos (0%). Our results suggest that NT embryos reconstructed with cells at the Cl phase have a high developmental competence from the time of embryo transfer to day 50 of gestation. (C) 2010 Elsevier B.V. All rights reserved.