Identification, characterization, and expression of dentin matrix protein 1 gene in Xenopus laevis.
Identification, characterization, and expression of dentin matrix protein 1 gene in Xenopus laevis.
复制标题
非洲爪蟾牙本质基质蛋白 1 基因的鉴定、表征和表达。
DOI：
10.1002/jez.b.22529
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
S. Shintani.
中科院分区：
文献类型：
--
作者：
T. Yonekura;H. Homma;A. Sakurai;M. Moriguchi;T. Miake;S. Toyosawa;S. Shintani.
牙本质基质蛋白1(DMP1)是一种酸性细胞外基质蛋白，主要在骨和牙本质中表达，是小整合素结合配体N-连接糖蛋白(SIPING)家族的成员。然而，与其他SIBLING基因相比，DMP1基因似乎进化得更快，尽管这些具有重要功能的分子通常比不太重要的分子进化得更慢。本研究的目的是鉴定和鉴定两栖动物中DMP1基因的一个同源基因，以阐明与四足类钙化组织相关的DMP1的分子进化变化。此外，我们还分析了该基因的mRNA表达，以阐明其在骨骼和牙胚发育中的功能变化，并与羊膜DMP1进行了比较。所推导的氨基酸序列的相似性。LevisDMP1与相应的羊膜蛋白相比很低，尽管它们确实有DMP1特有的几个独特特征，并具有相似的性质。X的表达式。Levis DMP1mRNA主要在骨细胞和成牙本质细胞中表达，但仅在成釉细胞中短暂表达，在羊膜细胞中也有表达。这些结果表明，DMP1在四足动物进化过程中具有几个保守的功能。这表明，在维持DMP1功能方面，生化特性的连续性比氨基酸残基序列的连续性更重要，氨基酸残基序列在分子进化过程中发生了变化。J.Exp.祖尔。(摩尔。戴夫。事件)320B：525-537,2013。©2013 Wiley期刊，Inc.
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix protein expressed mainly in bone and dentin, and is a member of the small integrin‐binding ligand N‐linked glycoprotein (SIBLING) family. TheDMP1gene, however, appears to evolve rapidly in comparison with otherSIBLINGgenes, even though such functionally important molecules usually evolve more slowly than less important ones. The purpose of this study was to identify and characterize an ortholog of theDMP1gene in an amphibian (Xenopus laevis;X. laevis) to clarify molecular evolutionary alterations in DMP1 associated with calcified tissues in tetrapods. Furthermore, we analyzed the mRNA expression of this gene to elucidate its functional change in bone and developing tooth germ in comparison with amniote DMP1s. The similarities of the deduced amino acid sequence ofX. laevisDMP1 to that of the corresponding amniote proteins were low, although they did share several unique features specific to DMP1 and have similar properties. Expression ofX. laevis DMP1mRNA was predominant in osteocytes and odontoblasts, but only transiently observed in ameloblasts, as in amniotes. These results suggest that DMP1 has conserved several functions during tetrapod evolution. This indicates that continuity of biochemical properties has been more important in maintaining DMP1 functionality than that of the sequence of amino acid residues, which has undergone change over the course of molecular evolution. J. Exp. Zool. (Mol. Dev. Evol.) 320B: 525–537, 2013. © 2013 Wiley Periodicals, Inc.