Structural and functional differentiation of a fat body-like tissue adhering to testis follicles facilitates spermatogenesis in locusts
Structural and functional differentiation of a fat body-like tissue adhering to testis follicles facilitates spermatogenesis in locusts
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附着在睾丸滤泡上的脂肪体样组织的结构和功能分化促进蝗虫的精子发生
DOI：
10.1016/j.ibmb.2019.103207
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发表时间：
2019
影响因子：
3.8
通讯作者：
Kang Le
中科院分区：
文献类型：
--
作者：
Ren Dani;Guo Wei;Yang Pengcheng;Song Juan;He Jing;Zhao Lianfeng;Kang Le
脂肪体分布在昆虫的全身，在中间代谢和营养储存中起着至关重要的作用。然而，脂肪体附着在不同组织上的分化功能在很大程度上仍不清楚。在这里，我们发现了一个围绕睾丸滤泡的脂肪体样组织，并从形态、细胞和分子水平描述了它的特征。其形态与腹部脂肪体不同，以二倍体细胞为主，而不是多倍体细胞。转录分析表明，Flt和Fb具有显著不同的基因表达谱。此外，细胞周期途径中的基因，包括DNA复制和细胞分裂相关基因，在Flt的发育过程中连续活跃，表明Flt细胞可能经历了有丝分裂周期而不是内周期。剥夺Flt可导致睾丸卵泡变形，精子束消失，精子总数减少，死亡精子增多，提示Flt在睾丸卵泡生精过程中起重要作用。这两种相似组织的特殊功能分化表明，Flt-Fb细胞能够建立一个很有前途的系统来研究有丝分裂到内周期的转变。
The fat body is distributed throughout the body of insects, playing the essential role in intermediary metabolism and nutrient storage. However, the function of differentiation of fat bodies adhering to different tissues remains largely unknown. Here, we identified a fat body-like tissue (FLT) surrounding testis follicles and described its features at morphological, cellular and molecular levels. The FLT is morphologically distinguished with the abdominal fat body (FB) and dominated by diploid cells instead of polyploid cells. The transcriptomic analysis demonstrated that the FLT and FB have dramatically different gene expression profiles. Moreover, genes in the cell cycle pathway, which include both DNA replication- and cell division-related genes, were successively active during development of the FLT, suggesting that FLT cells possibly undergo a mitotic cycle rather than an endocycle. Deprivation of the FLT resulted in distortion of the testis follicles, disappearance of sperm bundles, reduction of total sperm number and increase of dead sperm, indicating a critical role of the FLT in the spermatogenesis in testis follicles. The special functional differentiation of the two similar tissues suggested that FLT-FB cells are able to establish a promising system to study mitotic-to-endocycle transition.