Somatic Cell Encystment Promotes Abscission in Germline Stem Cells following a Regulated Block in Cytokinesis
Somatic Cell Encystment Promotes Abscission in Germline Stem Cells following a Regulated Block in Cytokinesis
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DOI：
10.1016/j.devcel.2015.05.003
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发表时间：
2015-07-27
影响因子：
11.8
通讯作者：
DiNardo, Stephen
中科院分区：
文献类型：
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作者：
Lenhart, Kari F.;DiNardo, Stephen
在许多组织中，干细胞龛必须协调多个干细胞谱系的行为。如何实现这一点在很大程度上是未知的。我们已经确定延迟完成的生殖干细胞（GSC）的胞质分裂作为一种机制，调节生产的干细胞的女儿在果蝇睾丸。通过实时成像，我们发现，通过调节Cofilin活性，在收缩环解体后形成次级F-肌动蛋白环，以阻止胞质分裂进程。这种阻断的持续时间由极光B激酶控制。此外，我们已经确定了体细胞包囊化的生殖细胞在促进GSC脱囊的要求。我们认为，这种非自主的作用促进了干细胞谱系之间的协调。这些发现揭示了GSC中胞质分裂被抑制和重新启动的机制，以及为什么这种复杂的调节存在于干细胞生态位中。
In many tissues, the stem cell niche must coordinate behavior across multiple stem cell lineages. How this is achieved is largely unknown. We have identified delayed completion of cytokinesis in germline stem cells (GSCs) as a mechanism that regulates the production of stem cell daughters in the Drosophila testis. Through live imaging, we show that a secondary F-actin ring is formed through regulation of Cofilin activity to block cytokinesis progress after contractile ring disassembly. The duration of this block is controlled by Aurora B kinase. Additionally, we have identified a requirement for somatic cell encystment of the germline in promoting GSC abscission. We suggest that this non-autonomous role promotes coordination between stem cell lineages. These findings reveal the mechanisms by which cytokinesis is inhibited and reinitiated in GSCs and why such complex regulation exists within the stem cell niche.