Diversity of N-acyl homoserine lactones in activated sludge detected by Fourier transform mass spectrometry
Diversity of N-acyl homoserine lactones in activated sludge detected by Fourier transform mass spectrometry
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DOI：
10.1038/s41545-019-0035-0
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发表时间：
2019-05
期刊：
影响因子：
11.4
通讯作者：
R. Honda;P. Phan;Tomohiro Tobino;S. Prasertkulsak;Smarch Panchavinin;Phuc-Nguon Hong;Chanreaksmey Taing;Manabu Noguchi;W. Chiemchaisri;C. Chiemchaisri
中科院分区：
文献类型：
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作者：
R. Honda;P. Phan;Tomohiro Tobino;S. Prasertkulsak;Smarch Panchavinin;Phuc-Nguon Hong;Chanreaksmey Taing;Manabu Noguchi;W. Chiemchaisri;C. Chiemchaisri
群体感应在污水生物处理过程中发挥着重要的作用。本研究旨在探讨N-酰基高丝氨酸内酯（阿勒）在废水生物处理工艺及其位置之间的差异。我们分析和定量的10阿勒活性污泥在污水处理厂（WWTPs）的傅立叶变换质谱（FTMS）和薄层色谱（TLC）的生物测定。在日本和泰国的七个污水处理厂，这是选择比较不同的工艺类型，包括中试规模的膜生物反应器工艺的污泥样品。在活性污泥中检测到各种阿勒，表明在许多实际的污水处理厂中存在的群体感应活性。LC-FTMS检测阿勒比TLC生物测定灵敏得多，因为TLC生物测定被暗示受活性污泥中群体淬灭分子的存在的影响。通过LC-FTMS分析，酰基中具有短直链的阿勒更丰富。特别是，C4-HSL，C7-HSL和C8-HSL被广泛检测到，与位置和工艺类型无关。AO工艺具有较高的阿勒生产率比其他工艺。
Quorum sensing is recently considered to play important roles in biological wastewater treatment processes. This study aimed to investigate diversity ofN-acyl homoserine lactones (AHL) among biological wastewater treatment processes and their locations. We analyzed and quantified 10 AHL in activated sludge at wastewater treatment plants (WWTPs) by Fourier transform mass spectrometry (FTMS) and by bioassay on thin-layer chromatography (TLC). Sludge samples were taken at seven WWTPs in Japan and in Thailand, which were chosen to compare different process types including a pilot-scale membrane bioreactor process. Various AHL were detected in activated sludge, suggesting the presence of quorum sensing activity in many practical WWTPs. Detection of AHL by LC-FTMS was much more sensitive than by TLC bioassay, because TLC bioassay was implied to be affected by the presence of quorum quenching molecules in activated sludge. With LC-FTMS analysis, AHL, which has short straight chain in the acyl group, were more abundant. Especially, C4-HSL, C7-HSL, and C8-HSL were detected widely independent of locations and process types. AO processes had higher AHL productivity than other processes.