INHIBITION OF SPONTANEOUS METASTASIS IN A RAT PROSTATE-CANCER MODEL BY ORAL-ADMINISTRATION OF MODIFIED CITRUS PECTIN
INHIBITION OF SPONTANEOUS METASTASIS IN A RAT PROSTATE-CANCER MODEL BY ORAL-ADMINISTRATION OF MODIFIED CITRUS PECTIN
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DOI：
10.1093/jnci/87.5.348
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发表时间：
1995-03-01
期刊：
影响因子：
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通讯作者：
RAZ, A
中科院分区：
文献类型：
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作者：
PIENTA, KJ;NAIK, H;RAZ, A
背景：前列腺癌是美国男性最常见的癌症，一旦转移就无法治愈。转移级联的许多阶段涉及细胞表面成分介导的细胞相互作用，如碳水化合物结合蛋白，包括半乳糖苷结合凝集素（galectin）。改性柑橘果胶（ph修饰）是一种从柑橘类水果中提取的植物纤维的可溶性成分，已被证明可以干扰细胞表面碳水化合物结合半乳糖凝集素-3分子介导的细胞间相互作用。目的：研究富含半乳糖残基的复合多糖修饰柑橘果胶对大鼠前列腺腺癌细胞自发转移的抑制作用。方法：采用cr -51标记法测定改性柑橘果胶对邓宁大鼠前列腺癌MAT-LyLu细胞粘附大鼠内皮细胞的抑制作用。通过琼脂糖中的集落形成，测定了改性柑橘果胶对MAT-LyLu细胞非锚定生长的抑制作用。免疫印迹和免疫组织化学证实了半乳糖凝集素-3在大鼠MAT-LyLu细胞和人前列腺癌中的存在。第0天，将100万个MAT-LyLu细胞皮下注射到雄性哥本哈根大鼠后肢。从第4天至第30天尸检，连续给予大鼠饮用水中0.0%、0.01%、0.1%或1.0% （wt/vol）改性柑橘果胶。计数肺中MAT-LyLu肿瘤菌落的数量。结果：与对照组第30天发生肺转移的16只大鼠中的15只相比，0.1%组14只大鼠中有7只、1.0%改性柑橘果胶组16只大鼠中有9只差异有统计学意义(双侧
Background: Prostate cancer is the most common cancer diagnosed in U.S. men and remains incurable once it has metastasized. Many stages of the metastatic cascade involve cellular interactions mediated by cell surface components, such as carbohydrate-binding proteins, including galactoside-binding lectins (galectins). Modified citrus pectin (pH-modified), a soluble component of plant fiber derived from citrus fruit, has been shown to interfere with cell-cell interactions mediated by cell surface carbohydrate-binding galectin-3 molecules. Purpose: The aim of this study was to determine whether modified citrus pectin, a complex polysaccharide rich in galactosyl residues, could inhibit spontaneous metastasis of prostate adenocarcinoma cells in the rat. Methods: The ability of modified citrus pectin to inhibit the adhesion of Dunning rat prostate cancer MAT-LyLu cells to rat endothelial cells was measured by Cr-51-labeling. Modified citrus pectin inhibition of MAT-LyLu cell anchorage-independent growth was measured by colony formation in agarose. The presence of galectin-3 in rat MAT-LyLu cells and human prostate carcinoma was demonstrated by immunoblotting and immunohistochemistry. One million MAT-LyLu cells were injected subcutaneously into the hind limb of male Copenhagen rats on day 0. Rats were given 0.0%, 0.01%, 0.1%, or 1.0% (wt/vol) modified citrus pectin continuously in their drinking water (from day 4 until necropsy on day 30). The number of MAT-LyLu tumor colonies in the lungs were counted. Results: Compared with 15 of 16 control rats that had lung metastases on day 30, seven of 14 rats in the 0.1% and nine of 16 rats in the 1.0% modified citrus-pectin group had statistically significant (two-sided; P