Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants
Cysteine synthase overexpression in tobacco confers tolerance to sulfur-containing environmental pollutants
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DOI：
10.1104/pp.126.3.973
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发表时间：
2001-07-01
期刊：
影响因子：
7.4
通讯作者：
Saito, K
中科院分区：
文献类型：
--
作者：
Noji, M;Saito, M;Saito, K
半胱氨酸（Cys）合酶[O-乙酰基-L-Ser（硫醇）-裂解酶，EC 4.2.99.8; CSase]负责Cys生物合成的最后一步。通过表达胞质CSase或叶绿体CSase的两个转化体的异受精产生了在胞质和叶绿体中具有增强的CSase活性的转基因烟草（Nicotiana tabacum; F-1）植物。F-1代转基因植株对有毒的二氧化硫和亚硫酸盐具有高度耐受性。经0.1 μ L穆尔L ~（-1）熏气处理后，F-1植株叶片中半胱氨酸和谷胱甘肽含量显著增加，而未转化对照植株叶片中半胱氨酸和谷胱甘肽含量没有显著增加。此外，F-1植株的叶片表现出对产生活性氧的除草剂百草枯的抗性增加。
Cysteine (Cys) synthase [O-acetyl-L-Ser(thiol)-lyase, EC 4.2.99.8; CSase] is responsible for the final step in biosynthesis of Cys. Transgenic tobacco (Nicotiana tabacum; F-1) plants with enhanced CSase activities in the cytosol and in the chloroplasts were generated by cross-fertilization of two transformants expressing cytosolic CSase or chloroplastic CSase. The F-1 transgenic plants were highly tolerant to toxic sulfur dioxide and sulfite. Upon fumigation with 0.1 muL L-1 sulfur dioxide, the Cys and glutathione contents in leaves of F-1 plants were increased significantly, but not in leaves of non-transformed control plants. Furthermore, the leaves of F-1 plants exhibited the increased resistance to paraquat, a herbicide generating active oxygen species.