Matrix vapor deposition/recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ionization imaging mass spectrometry (SMALDI-MS) of tissue and single cells
Matrix vapor deposition/recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ionization imaging mass spectrometry (SMALDI-MS) of tissue and single cells
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DOI：
10.1002/rcm.4401
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Spengler, Bernhard
中科院分区：
文献类型：
--
作者：
Bouschen, Werner;Schulz, Oliver;Spengler, Bernhard
基质制备技术，如空气喷雾或气相沉积进行了研究，相对于横向迁移，集成的分析物到矩阵晶体和可实现的横向分辨率的目的，高分辨率生物成像。可访问的质量范围被认为是超过5000与足够的分析灵敏度。气体辅助喷雾方法（使用无氧气体）在分析物的晶体整合和样品内的分析物迁移之间提供了良好的折衷。然而，用这种方法控制结晶参数是困难的。相反，将制备过程分为两个步骤导致对迁移和掺入的改进控制。第一步是将基质干燥气相沉积到所研究的样品上。在第二步中，通过在饱和水气氛中控制基质的重结晶来增强分析物掺入基质晶体中。用后一种方法实现了有效的分析分辨率为2 μ m的x和y方向的扫描微探针基质辅助激光解吸/电离成像质谱（SMALDI-MS）。采用这种新的制备技术，成功地对培养的人肾癌A-498细胞进行了高分辨MALDI成像研究。版权所有（C）2010约翰威利父子有限公司
Matrix preparation techniques such as air spraying or vapor deposition were investigated with respect to lateral migration, integration of analyte into matrix crystals and achievable lateral resolution for the purpose of high-resolution biological imaging. The accessible mass range was found to be beyond 5000 a with sufficient analytical sensitivity. Gas-assisted spraying methods (using oxygen-free gases) provide a good compromise between crystal integration of analyte and analyte migration within the sample. Controlling preparational parameters with this method, however, is difficult. Separation of the preparation procedure into two steps, instead, leads to an improved control of migration and incorporation. The first step is a dry vapor deposition of matrix onto the investigated sample. In a second step, incorporation of analyte into the matrix crystal is enhanced by a controlled recrystallization of matrix in a saturated water atmosphere. With this latter method an effective analytical resolution of 2 mu m in the x and y direction was achieved for scanning microprobe matrix-assisted laser desorption/ionization imaging mass spectrometry (SMALDI-MS). Cultured A-498 cells of human renal carcinoma were successfully investigated by high-resolution MALDI imaging using the new preparation techniques. Copyright (C) 2010 John Wiley & Sons, Ltd.