A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources
A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources
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Aurantiochytrium limacinum SR21以葡萄糖和甘油为混合碳源高效生产二十二碳六烯酸的策略
DOI：
10.1016/j.biortech.2014.11.046
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发表时间：
2015-02-01
影响因子：
11.4
通讯作者：
Wang, Xingguo
中科院分区：
文献类型：
--
作者：
Li, Jing;Liu, Ruijie;Wang, Xingguo
葡萄糖和甘油是培养金氧化钇SR21的有效碳源。葡萄糖促进A. limacinum SR21的快速生长和脂质合成，甘油促进二十二碳六烯酸（DHA）的积累。为了提高A. limacinum SR21的DHA产量，以葡萄糖和甘油为混合碳源（MCSs）进行摇瓶培养和补料分批培养。通过对MCSs的优化，在恒定供气条件下分批补料发酵的DHA产量和产率最高，分别为32.36 g/L和337.1 mg/L/h。与以葡萄糖为单碳源（SCS）相比，其DHA产量提高了15.24%。本研究提出了一种高效、经济的产DHA策略。(C) 2014 Elsevier Ltd.版权所有。
Glucose and glycerol are useful carbon sources for the cultivation of Aurantiochytrium limacinum SR21. Glucose facilitates rapid growth and lipid synthesis, and glycerol promotes the accumulation of docosahexaenoic acid (DHA) in A. limacinum SR21. To improve the DHA productivity of A. limacinum SR21, shake flask and fed-batch cultures were performed using glucose and glycerol as mixed carbon sources (MCSs). Along with optimization of the MCSs, the best DHA yield and productivity (32.36 g/L and 337.1 mg/L/h) were obtained via fed-batch fermentation with maintenance of a constant air supply. The DHA productivity was 15.24% higher than that obtained using glucose as single carbon source (SCS). This study presents a highly efficient and economic strategy for the production of DHA by A. limacinum SR21. (C) 2014 Elsevier Ltd. All rights reserved.