Response of aorta connective tissue matrix to injury caused by vassopressin-induced hypertension or hypercholesterolemia.
Response of aorta connective tissue matrix to injury caused by vassopressin-induced hypertension or hypercholesterolemia.
复制标题
主动脉结缔组织基质对加压素诱导的高血压或高胆固醇血症引起的损伤的反应。
DOI：
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发表时间：
2000
影响因子：
2.2
通讯作者：
J. Lorenc
中科院分区：
文献类型：
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作者：
J. Drobnik;R. Dąbrowski;A. Szczepanowska;L. Giernat;J. Lorenc
本研究的目的是研究两种不同的致动脉粥样硬化刺激(血管加压素诱导的高血压或高胆固醇血症)对胸、腹主动脉内外段胶原和糖胺聚糖(GAG)含量的影响。实验兔分为4组：对照组、实验第1~2 5天分别注射生理盐水或加压素1IU/kg组。第4组动物饲喂含胆固醇0.25%的饲料。只有加压素治疗组的收缩压从研究开始时的110毫米汞柱上升到研究结束时的166毫米汞柱。14周后，将大鼠的主动脉分离为内外两部分。GAG组分被分离并鉴定为糖醛酸。用组织中羟脯氨酸含量来评价胶原蛋白的含量。高胆固醇血症或高血压兔胸主动脉内膜总GAG和硫酸乙酰肝素(HS)水平升高，而胶原含量无明显变化。在高血压动物中，这些变化延伸到主动脉的外部，此外，还包括软骨素-4硫酸盐(C-4S)含量的升高。两种致动脉粥样硬化刺激可增加腹主动脉胶原水平，但不增加腹主动脉GAG含量。高血压或高胆固醇血症所致的损伤对兔主动脉各部位的胶原、总GAG和HS含量均有趋同作用。在高血压和高胆固醇血症兔中，常见的GAG在胸主动脉中增加，代表HS。由于动脉不同节段对动脉粥样硬化发展的敏感性不同，结缔组织基质对高血压或高胆固醇血症引起的损伤表现出不同的反应。
The aim of the study was to investigate the effect of two various atherogenic stimuli (vasopressin-induced hypertension or hypercholesterolemia) on the collagen and glycosaminoglycan (GAG) content in the internal or external part of both thoracic and abdominal aorta, which are differently susceptible to atherosclerosis. Experimental rabbits were divided into four groups: controls, animals injected with physiological saline or vasopressin at the dose of 1 IU/kg from the 1 st to the 25 th day of experiment, respectively. The animals from group 4 were maintained on food, containing 0.25% cholesterol. Only in the vasopressin-treated group, the systolic blood pressure was elevated from 110 mmHg at the beginning, to 166 mmHg at the end of the study. After 14 weeks the aorta was dissected into internal and external parts. GAG fractions were separated and estimated as uronic acids. Collagen was evaluated as the hydroxyproline content in the tissue. Augmented total GAG and heparan sulphate (HS) level, plus no changes in the collagen content were seen in the internal part of the thoracic aorta in rabbits with hypercholesterolemia or hypertension. In the hypertensive animals, the changes were extended to the external part of the aorta and, additionally, comprised the elevation of the chondroitin-4 sulphate (C-4S) content. The two atherogenic stimuli increased the collagen level with no elevation of the GAG content in the abdominal aorta. A convergent effect of the injury, caused by hypertension or hypercholesterolemia on the collagen, total GAG and HS content was shown in the respective parts of the rabbit aortas. The common GAG, increased in the thoracic aorta, stand for the HS, in both hypertensive and hypercholesterolemic rabbits. As the sensitivity to atherosclerosis development in different segments of the aorta varies, they express various responses of the connective tissue matrix to injuries, caused by hypertension or hypercholesterolemia.