Comparative analysis of hmuO function and expression in Corynebacterium species
Comparative analysis of hmuO function and expression in Corynebacterium species
复制标题
DOI：
10.1128/jb.00056-07
复制
发表时间：
2007-05-01
影响因子：
3.2
通讯作者：
Schmitt, Michael P.
中科院分区：
文献类型：
--
作者：
Kunkle, Carey A.;Schmitt, Michael P.
我们在白喉棒状杆菌和溃疡棒状杆菌的hmuO基因中构建了确定的缺失，并表明C。ulcerans hmuO突变导致血红蛋白-铁利用率显著降低，而C.白喉菌株中，hmuO的缺失导致血红素作为铁源的利用没有或仅部分减少。我们还证明了C. ulcerans hmuO启动子受铁和血红素的调控最小，而C.白喉hmuO启动子显示出显著的铁抑制和血红素依赖性激活。这些发现表明在棒状杆菌属物种中存在HmuO功能和表达的变异性。
We have constructed defined deletions in the hmuO gene from Corynebacterium diphtheriae and Corynebacterium ulcerans and show that the C. ulcerans hmuO mutation results in a significant reduction in hemoglobin-iron utilization, whereas in C. diphtheriae strains, deletion of hmuO caused no or only partial reduction in the utilization of heme as an iron source. We also show that expression from the C. ulcerans hmuO promoter exhibits minimal regulation by iron and heme whereas transcription from the C. diphtheriae hmuO promoter shows both significant iron repression and heme-dependent activation. These findings indicate that variability in HmuO function and expression exists among Corynebacterium species.