LYMPHOID GERMINAL-CENTERS ARE RESERVOIRS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA
LYMPHOID GERMINAL-CENTERS ARE RESERVOIRS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA
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DOI：
10.1093/infdis/164.6.1051
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发表时间：
1991-12-01
影响因子：
6.4
通讯作者：
FAUCI, AS
中科院分区：
文献类型：
--
作者：
FOX, CH;TENNERRACZ, K;FAUCI, AS
当放射性标记的RNA用于原位杂交，人类免疫缺陷病毒1型（HIV-1）的RNA被发现在高浓度的生发中心的淋巴组织从HIV-1感染的患者。来自杂交探针的大部分信号不依赖于特定细胞，在疾病控制中心（CDC）11级和疾病的成人患者或疾病控制中心P-2A级疾病的儿科患者检查的所有淋巴组织中，均在生发中心的细胞外间隙中发现。来自CDC IV类感染的成人患者或CDC P-2D类疾病的儿科患者的类扁桃体组织（包括尸检材料）缺乏完整的生发中心，并且HIV-1 RNA仅在罕见的分离细胞中发现，一些组织没有检测到HIV-1 RNA。因此，在HIV感染的早期阶段，生发中心是间质空间中游离病毒的重要储存库，随着疾病的进展，生发中心逐渐消失。
When radiolabeled RNA was used for in situ hybridization, human immunodeficiency virus type 1 (HIV-1) RNA was found in high concentrations in germinal centers of lymphoid tissues from patients with HIV-1 infection. Most of the signal from hybridized probe was independent of specific cells, being found in the extracellular space of germinal centers in all lymphoid tissues examined from adult patients with Centers for Disease Control (CDC) class 11 and ill disease or pediatric patients with CDC class P-2A disease. Lymphoid tissues from adult patients with CDC class IV infections or pediatric patients with CDC class P-2D disease (including autopsy material) lacked intact germinal centers, and HIV-1 RNA was then found only in rare, isolated cells, with some tissues having no detectable HIV-1 RNA. Thus, in the early stages of HIV infection, germinal centers serve as important reservoirs of free virus in the interstitial spaces, and this reservoir disappears as the germinal centers involute with advancing disease.