Effect and Mechanism of Ginsenosides CK and Rg1 on Stimulation of Glucose Uptake in 3T3-L1 Adipocytes
Effect and Mechanism of Ginsenosides CK and Rg1 on Stimulation of Glucose Uptake in 3T3-L1 Adipocytes
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DOI：
10.1021/jf9034755
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发表时间：
2010-05-26
影响因子：
6.1
通讯作者：
Chang, Tsu-Chung
中科院分区：
文献类型：
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作者：
Huang, Yu-Chuan;Lin, Cheng-Yu;Chang, Tsu-Chung
在373-L1脂肪细胞中研究了黄芪皂苷化合物K（OK）和Rg 1的葡萄糖调节活性。两种化合物均以剂量反应方式显著增强3 T3-L1脂肪细胞中的葡萄糖摄取，这与脂肪细胞中GLUT 4从细胞内囊泡向质膜的转位增加相关。OK和Rg 1对葡萄糖摄取和GLUT 4易位的刺激作用与AMP活化蛋白激酶（AMPK）和磷脂酰肌醇3-激酶（PI 3 K）信号通路的激活有关;这两条通路都是介导动物细胞葡萄糖摄取的关键通路。除了葡萄糖摄取的急性刺激作用外，CK和Rg 1的长时间孵育显著诱导3 T3-L1脂肪细胞在mRNA和蛋白水平上的GLUT 4表达，而不是GLUT 1。进一步的研究表明，OK抑制和Rg 1增强脂肪细胞中甘油三酯的积累，这表明这些皂苷的作用机制可能并不完全相同。综上所述，本研究证明了CK和Rg 1在脂肪细胞中的胰岛素样活性。这些发现对于了解人参和人参皂苷的降血糖特性和潜在应用具有重要意义。
The glucoregulatory activities of ginsenosides compound K (OK) and Rg1 were investigated in 373-L1 adipocytes. Both compounds significantly enhanced glucose uptake in 3T3-L1 adipocytes in a dose response manner, which is correlated with increased GLUT4 translocation from intracellular vesicles to the plasma membrane in adipocytes. The stimulating effects of OK and Rg1 on glucose uptake and GLUT4 translocation are associated with activation of AMP-activated protein kinase (AMPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways; both are key pathways in mediating glucose uptake in animal cells. In addition to the acute stimulus effect of glucose uptake, prolonged incubation of CK and Rg1 significantly induced GLUT4, but not GLUT1, expression at both the mRNA and protein levels in 3T3-L1 adipocytes. Further studies showed that OK inhibited and Rg1 enhanced triglyceride accumulation in adipocytes, suggesting that the mechanism of action of these ginsenosides may not be completely identical. In summary, this study demonstrated insulin-like activities of CK and Rg1 in adipocytes. These findings are important in understanding the hypoglycemic properties and potential applications of ginseng and ginsenosides.