Mother-to-embryo vitellogenin transport in a viviparous teleost Xenotoca eiseni
Mother-to-embryo vitellogenin transport in a viviparous teleost Xenotoca eiseni
复制标题
DOI：
10.1073/pnas.1913012116
复制
发表时间：
2019-10-29
影响因子：
11.1
通讯作者：
Sano, Kaori
中科院分区：
文献类型：
--
作者：
Iida, Atsuo;Arai, Hiroyuki N.;Sano, Kaori
卵黄蛋白原（Vtg）是一种卵黄营养蛋白，在雌性动物的肝脏中合成，随后被携带到卵巢中，有助于卵生动物的卵黄发生。因此，Vtg水平在卵子发生期间升高。相反，Vtg蛋白在胎生哺乳动物中已经遗传丢失，因此卵黄蛋白不参与它们的卵子发生和胚胎发育。在这项研究中，我们确定了Vtg蛋白在妊娠期间的胎生硬骨鱼，Xenotoca艾森尼女性的肝脏。虽然卵黄发生在妊娠期间被捕，生化分析表明，Vtg蛋白存在于卵巢组织和腔液。Vtg蛋白在卵巢内胚胎的滋养带中也有表达。免疫电镜显示，Vtg蛋白被吸收到细胞内的囊泡在上皮细胞的滋养层。此外，随后在卵巢内胚胎的滋养层中检测到注射到妊娠雌性腹腔中的外源性Vtg蛋白。我们的数据表明，卵黄蛋白是X染色体妊娠期间母体向卵巢内胚胎提供的营养因子之一。爱生。
Vitellogenin (Vtg), a yolk nutrient protein that is synthesized in the livers of female animals, and subsequently carried into the ovary, contributes to vitellogenesis in oviparous animals. Thus, Vtg levels are elevated during oogenesis. In contrast, Vtg proteins have been genetically lost in viviparous mammals, thus the yolk protein is not involved in their oogenesis and embryonic development. In this study, we identified Vtg protein in the livers of females during the gestation of the viviparous teleost, Xenotoca eiseni. Although vitellogenesis is arrested during gestation, biochemical assays revealed that Vtg protein was present in ovarian tissues and lumen fluid. The Vtg protein was also detected in the trophotaeniae of the intraovarian embryo. Immunoelectron microscopy revealed that Vtg protein is absorbed into intracellular vesicles in the epithelial cells of the trophotaeniae. Furthermore, extraneous Vtg protein injected into the abdominal cavity of a pregnant female was subsequently detected in the trophotaeniae of the intraovarian embryo. Our data suggest that the yolk protein is one of the matrotrophic factors supplied from the mother to the intraovarian embryo during gestation in X. eiseni.