Upregulation of Lhx8 increase VAChT expression and ACh release in neuronal cell line SHSY5Y
Upregulation of Lhx8 increase VAChT expression and ACh release in neuronal cell line SHSY5Y
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Lhx8 上调可增加神经元细胞系 SHSY5Y 中 VAChT 表达和 ACh 释放
DOI：
10.1016/j.neulet.2013.11.047
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发表时间：
2014-01-24
影响因子：
2.5
通讯作者：
Qin, Jianbing
中科院分区：
文献类型：
--
作者：
Li, Haoming;Jin, Guohua;Qin, Jianbing
Lhx 8是胆碱能分化的转录因子。我们先前的实验发现Lhx 8的上调促进海马神经干/祖细胞或海马新生神经元的胆碱能神经元分化。然而，Lhx 8在VAChT表达和ACh释放中的作用仍然不太清楚。本研究利用慢病毒载体将Lhx 8基因转染神经元细胞株SHSY 5 Y，获得了稳定高表达Lhx 8的细胞。利用此细胞模型，我们提供了实验证据，增加Lhx 8上调ChAT和VAChT的表达，也增加了ACh释放在培养基中。我们认为Lhx 8过表达是一种增加ACh释放的有效策略，可能对神经退行性疾病具有治疗价值。(c)2013爱思唯尔爱尔兰有限公司版权所有。
Lhx8 is a transcription factor for cholinergic differentiation. Our previous experiments found upregulation of Lhx8 promoted cholinergic neuronal differentiation of hippocampal neural stem/progenitor cells or hippocampal newborn neurons in vitro. However, the role of Lhx8 in VAChT expression and ACh release is still less understood. In this report, we transfected Lhx8 cDNA into neuronal cell line SHSY5Y by lentiviral vectors to acquire the cells which stably expressed high level of Lhx8. Using this cell model, we provided experimental evidence that increasing Lhx8 upregulated the expression of ChAT and VAChT, and also increased the ACh release in culture medium. We suggested that Lhx8 overexpression is a useful strategy to increase the release of ACh and maybe of therapeutic value to neurodegenerative diseases. (c) 2013 Elsevier Ireland Ltd. All rights reserved.