Spermatological characters of the digenean Rubenstrema exasperatum (Rudolphi, 1819) (Plagiorchioidea, Omphalometridae)
Spermatological characters of the digenean Rubenstrema exasperatum (Rudolphi, 1819) (Plagiorchioidea, Omphalometridae)
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DOI：
10.1007/s00436-010-2178-2
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发表时间：
2011-05-01
影响因子：
2
通讯作者：
Miquel, Jordi
中科院分区：
文献类型：
--
作者：
Bakhoum, Abdoulaye J. S.;Tidiane Ba, Cheikh;Miquel, Jordi
我们首次对Rubenstrema exasperatum（Omphalometridae）的精子发生和精子进行超微结构描述，Rubenstrema exasperatum（Omphalometridae）是Sorex araneus（Soricidae）的一种肠道寄生虫。精子发生始于分化区的形成，分化区的底部由拱形膜环界定，并以皮质微管为边界。该区域包含两个与横纹细根和中心粒间体相关的中心粒。它还含有细胞核和大量线粒体。中心粒间体由七个电子致密层组成。两个中心粒产生两个游离鞭毛，其垂直于中值细胞质突起生长。此外，鞭毛旋转之后是鞭毛与正中胞质突的近远端融合，而细胞核和线粒体沿着精子细胞迁移。拱形膜环的收缩产生了年轻的精子。 R. exasperatum 的成熟精子显示出双殖系精子中发现的几种超微结构特征，如两个轴丝、线粒体、两束平行的皮质微管、细胞核和糖原颗粒。该物种的精子中也存在质膜和刺状体的外部装饰。在本研究中，我们产生了有关先前未探索的家族的额外精子学数据，并且我们还将我们的数据与Digenea中现有的超微结构描述进行比较，以评估提议作为系统发育工具的几个特征的使用。
We present the first ultrastructural description of spermiogenesis and of the spermatozoon of Rubenstrema exasperatum (Omphalometridae), an intestinal parasite of Sorex araneus (Soricidae). Spermiogenesis begins with the formation of the differentiation zone delimited at the base by the ring of the arched membranes and bordered by cortical microtubules. This area contains two centrioles associated with striated rootlets and with an intercentriolar body. It also contains the nucleus and numerous mitochondria. The intercentriolar body is made up of seven electron-dense layers. The two centrioles give rise to two free flagella that grow orthogonally to the median cytoplasmic process. Additionally, flagellar rotation is followed by the proximodistal fusion of the flagella with the median cytoplasmic process, while the nucleus and mitochondria migrate along the spermatid. The constriction of the ring of arched membranes gives rise to the young spermatozoon. The mature spermatozoon of R. exasperatum shows several ultrastructural characters found in digenean spermatozoa such as two axonemes, mitochondrion, two bundles of parallel cortical microtubules, nucleus, and granules of glycogen. External ornamentation of the plasma membrane and spinelike bodies are also present in the spermatozoon of this species. In the present study, we produced additional spermatological data concerning a previously unexplored family, and we also compare our data to the existent ultrastructural descriptions within the Digenea in order to assess the use of several characters proposed as phylogenetic tools.