Apelin and its receptor control heart field formation during zebrafish gastrulation
Apelin and its receptor control heart field formation during zebrafish gastrulation
复制标题
DOI：
10.1016/j.devcel.2007.01.011
复制
发表时间：
2007-03-01
影响因子：
11.8
通讯作者：
Solnica-Krezel, Lilianna
中科院分区：
文献类型：
--
作者：
Zeng, Xin-Xin I.;Wilm, Thomas P.;Solnica-Krezel, Lilianna
脊椎动物的心脏在原肠胚形成期间出现，心脏前体从侧板中胚层区域向胚胎中线会聚，并向嘴侧延伸形成双侧心脏野。G蛋白偶联受体（GPCRs）介导神经和免疫系统的功能;然而，它们在原肠胚形成中的作用在很大程度上尚未探索。在这里，我们表明，斑马鱼同源的Agtril 1b受体及其配体，爱帕琳，牵连在生理学和血管生成，控制心脏领域的形成。斑马鱼原肠胚在侧板中胚层表达agtrl 1b，而apelin表达仅限于中线。Agtril 1b或Apelin功能的减少或过量导致心脏前体和随后的心脏缺乏。在Apelin缺陷的原肠胚，心脏前体聚集无效的心脏领域，并表现出异位分布，而心脏前体过表达Apelin表现出异常的形态和喙迁移。我们的研究结果牵连GPCR信号在运动的离散细胞群，建立器官雏形在脊椎动物原肠胚。
The vertebrate heart arises during gastrulation as cardiac precursors converge from the lateral plate mesoderm territories toward the embryonic midline and extend rostrally to form bilateral heart fields. G protein-coupled receptors (GPCRs) mediate functions of the nervous and immune systems; however, their roles in gastrulation remain largely unexplored. Here, we show that the zebrafish homologs of the Agtril1b receptor and its ligand, Apelin, implicated in physiology and angiogenesis, control heart field formation. Zebrafish gastrulae express agtrl1b in the lateral plate mesoderm, while apelin expression is confined to the midline. Reduced or excess Agtril1b or Apelin function caused deficiency of cardiac precursors and, subsequently, the heart. In Apelin-deficient gastrulae, the cardiac precursors converged inefficiently to the heart fields and showed ectopic distribution, whereas cardiac precursors overexpressing Apelin exhibited abnormal morphology and rostral migration. Our results implicate GPCR signaling in movements of discrete cell populations that establish organ rudiments during vertebrate gastrulation.