Fibronectin-like immunoreactivity in Helisoma buccal ganglia: evidence that an endogenous fibronectin-like molecule promotes neurite outgrowth.
Fibronectin-like immunoreactivity in Helisoma buccal ganglia: evidence that an endogenous fibronectin-like molecule promotes neurite outgrowth.
复制标题
Helisoma 颊神经节中的纤连蛋白样免疫反应性：内源性纤连蛋白样分子促进神经突生长的证据。
DOI：
10.1002/neu.480190306
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Kater,SB
中科院分区：
文献类型：
--
作者：
Mattson,MP;Kater,SB
我们研究了与完整颊神经节、细胞培养的颊神经节神经元和非神经元细胞以及蜗牛海鞘瘤脑条件培养液相关的纤维连接素样(FNL)免疫反应的分布。此外，还研究了纤维连接蛋白在神经突起生长调控中的可能作用。FNL抗原的免疫荧光染色显示，颊神经节和神经干的结缔组织鞘上的斑块和纤维阵列有强烈的染色。在神经节内，在神经元周围和轨道状排列中可见浓染。在细胞培养中，特异性染色与非神经元细胞表面有关，与已鉴定神经元表面的联系程度较小。此外，脑条件培养液中的一个非细胞、底物结合成分显示出FNL免疫反应。由于培养的螺旋动脉瘤需要一种底物相关的脑源性条件因子(CF)来形成具有运动生长锥体的神经突起，我们测试了FNL免疫反应物质是否可能起到神经趋化作用。纤维连接蛋白抗血清以剂量依赖的方式抑制CF诱导的已鉴定神经元在分离细胞培养中的萌发。当纤维连接蛋白抗血清以更高的浓度加入已经对CF反应生长的神经元时，对轴突的延长起到双相作用；在低浓度时促进突起的生长，在高浓度时抑制突起的生长。相反，随着抗血清水平的增加，与运动性相关的生长锥体结构(丝足和片脂)逐渐减少。来自纤维连接蛋白细胞结合域的短肽(Arg-Gly-Asp-Ser)也大大减少了轴突的生长。这项研究的综合结果表明，在Helisoma的CNS中有丰富的FNL免疫反应分子，它们可能是由非神经细胞产生的，它们作为CF底物相关成分，促进生长锥丝节和板脂节的活动。
We examined the distribution of fibronectin‐like (FNL) immunoreactivity associated with intact buccal ganglia, cell‐cultured buccal ganglia neurons and nonneuronal cells, and brain‐conditioned medium from the snailHelisoma. In addition, the possible roles of fibronectin in the regulation of neurite outgrowth were studied. Immunofluorescent staining for FNL antigens revealed intense staining in patches and fibrous arrays over the connective tissue sheaths of buccal ganglia and nerve trunks. Within the ganglia, heavy staining was seen surrounding neurons and in track‐like arrangements. In cell cultures, specific staining was associated with nonneuronal cell surfaces and to a lesser degree with the surface of identified neurons. In addition, a noncellular, substrate‐bound component of brain‐conditioned medium displayed FNL immunoreactivity. Since culturedHelisomaneurons require a substrate‐associated, brain‐derived conditioning factor (CF) in order to elaborate neurites with motile growth cones, we tested whether the FNL immunoreactive substance might act as a neuritotropic agent. Fibronectin antiserum suppressed, in a dose‐dependent manner, the CF‐induced sprouting of identified neurons in isolated cell culture. When added at increasing concentrations to neurons already growing in response to CF, fibronectin antiserum exerted a biphasic effect on neurite elongation; outgrowth was accelerated at low, but inhibited at high, antiserum concentrations. In contrast, growth cone structures associated with motility (filopodia and lamellipodia) were progressively reduced by increasing levels of antiserum. A short peptide derived from fibronectin's cell‐binding domain (Arg‐Gly‐Asp‐Ser) also greatly reduced neurite outgrowth. The combined results of this study indicate an abundance of FNL immunoreactive molecules within the CNS ofHelisoma, their probable production by nonneuronal cells, and their function as a substrate‐associated component of CF which promotes growth cone filopodial and lamellipodial activity.