Cytocompatibility of self-assembled ß-hairpin peptide hydrogel surfaces
Cytocompatibility of self-assembled ß-hairpin peptide hydrogel surfaces
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DOI：
10.1016/j.biomaterials.2005.01.029
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Schneider, JP
中科院分区：
文献类型：
--
作者：
Kretsinger, JK;Haines, LA;Schneider, JP
MAX 1是一种20个氨基酸的肽，其经历触发自组装以形成刚性水凝胶。当溶解在水溶液中时，该肽以无规卷曲构象的集合存在，使其完全可溶。外源刺激的加入导致肽折叠成β-发夹构象。这种折叠结构经历快速组装成高度交联的水凝胶网络。DMEM细胞培养基是一种能够引发MAX 1折叠和随后自组装的刺激物。证明了该凝胶对NIH 3 T3鼠成纤维细胞的细胞相容性。凝胶显示对成纤维细胞无毒。MAX 1水凝胶还促进细胞在血清蛋白存在或不存在的情况下附着于水凝胶支架的能力。此外，MAX 1水凝胶能够支持成纤维细胞增殖至融合，而对支架的流变学性质几乎没有影响。MAX 1水凝胶满足组织工程支架的初步机械和细胞相容性要求。(c)2005爱思唯尔有限公司保留所有权利。
MAX1 is a 20 amino acid peptide that undergoes triggered self-assembly to form a rigid hydrogel. When dissolved in aqueous solutions, this peptide exists in an ensemble of random coil conformations rendering it fully soluble. The addition of an exogenous stimulus results in peptide folding into beta-hairpin conformation. This folded structure undergoes rapid assembly into a highly crosslinked hydrogel network. DMEM cell culture media is one stimulus able to initiate folding and consequent self-assembly of MAX1. The cytocompatibility of this gel towards NIH 3T3 murine fibroblasts is demonstrated. Gels were shown to be non-toxic to the fibroblast cells. MAX1 hydrogels also foster the ability of the cells to attach to the hydrogel scaffold in the absence or presence of serum proteins. Additionally MAX1 hydrogels were able to support fibroblast proliferation to confluency with little effect on the rheological properties of the scaffold. MAX1 hydrogels meet the preliminary mechanical and cytocompatibiltiy requirements of a tissue engineering scaffold. (c) 2005 Elsevier Ltd. All rights reserved.