Effect of hydrogen-rich saline on apoptosis induced by hepatic ischemia reperfusion upon laparoscopic hepatectomy in miniature pigs
Effect of hydrogen-rich saline on apoptosis induced by hepatic ischemia reperfusion upon laparoscopic hepatectomy in miniature pigs
复制标题
富氢盐水对小型猪腹腔镜肝切除肝缺血再灌注诱导细胞凋亡的影响
DOI：
10.1016/j.rvsc.2018.07.005
复制
发表时间：
2018
影响因子：
2.4
通讯作者：
Wang Hongbin
中科院分区：
文献类型：
--
作者：
Zhang Qianzhen;Ge Yansong;Li Hui;Bai Ge;Jiao Zhihui;Kong Xiangdong;Meng Weijing;Wang Hongbin
肝缺血再灌注损伤(HIRI)是肝外科手术和肝移植中常见的损伤。氢是一种安全有效的抗氧化剂，对肝脏损伤具有保护作用。在这项研究中，我们研究了富氢盐水(HRS)在小型猪肝切除术后腹腔镜性HIRI模型中对细胞凋亡的作用。将巴马小型猪随机分为假手术组、I/R组和HRS组。再灌流前10min、术后1d、2d、3d门静脉注射HRS 10m L/kg。结果表明，HRS治疗可显著降低血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)活性和TUNEL阳性细胞数。经HRS处理后，逆转录聚合酶链式反应(RT-qPCR)和免疫印迹法检测到P53、Bax基因和蛋白的表达明显降低，而bcl2基因的基因和蛋白表达明显增强。此外，HRS治疗后Caspase-3和Caspase-9活性显著降低。结论：HRS可通过抑制细胞凋亡而减轻小型猪HIRI和肝切除术后的肝损伤，改善肝功能。
Hepatic ischemia reperfusion injury (HIRI) occurs commonly in liver surgery and liver transplantation. Hydrogen, a safe and effective antioxidant, exerts a protective effect against liver injury. In this study, we investigated the role of hydrogen-rich saline (HRS) in apoptosis in a miniature pig model of laparoscopic HIRI upon hepatectomy. Bama miniature pigs were randomly assigned to sham, I/R and HRS groups. The pigs received 10 mL/kg HRS by portal venous injection 10 min before reperfusion and at 1 d, 2 d, and 3 d after surgery. The results showed that HRS treatment significantly decreased serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) activity and TUNEL-positive cells. Upon HRS treatment, the expression of P53 and Bax mRNA and protein by RT-qPCR and Western blot was markedly decreased, whereas the expression of bcl-2 mRNA and protein was significantly increased. Moreover, Caspase-3 and Caspase-9 activities were significantly decreased upon treatment with HRS. In conclusion, the results indicate that HRS could alleviate liver injury and improve liver function via inhibiting apoptosis after laparoscopic HIRI and hepatectomy injury in miniature pigs.