A progestin regulates the prostaglandin pathway in the neuroendocrine system in female mudskipper Boleophthalmus pectinirostris
A progestin regulates the prostaglandin pathway in the neuroendocrine system in female mudskipper Boleophthalmus pectinirostris
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DOI：
10.1016/j.jsbmb.2023.106300
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发表时间：
2023-04-11
影响因子：
4.1
通讯作者：
Chen，Shi Xi
中科院分区：
文献类型：
--
作者：
Ma，He;Hong，Wang Shu;Chen，Shi Xi
性激素通过脑垂体轴调控生殖周期，但其分子机制仍不清楚。大弹涂鱼（Boleophthalmus pectinirostris）在生殖季节具有半月周期性产卵节律，这与硬骨鱼类性激素17α，20 β-二羟基-4-二氢-3-酮（DHP）的前体17α-羟孕酮的半月周期性变化相一致。在本研究中，我们使用RNA-seq在体外研究了DHP处理组织和对照组之间的脑转录差异。差异表达分析显示2700个基因表达显著差异，其中上调基因1532个，下调基因1168个。大多数前列腺素途径相关基因显著上调，特别是前列腺素受体6（ptger 6）。原位杂交结果表明，ptger 6基因在大鼠端脑腹侧区、端脑腹侧区腹侧核、小细胞视前核前部、大细胞视前核大细胞部、下丘脑室周围腹侧区、前结节核、后结节室周核和枕。DHP通过Pgr显著增强ptger 6的启动子活性。总之，这项研究表明，DHP调节前列腺素途径的神经内分泌系统的硬骨鱼。
Sex hormones regulate the reproductive cycle through brain-pituitary axis, but the molecular mechanism is still enigmatic. In the reproductive season, the mudskipperBoleophthalmus pectinirostrispossesses a semilunar periodicity spawning rhythm, which coincides with the semilunar periodicity variations in 17α-hydroxyprogesterone, the precursor of 17α,20β-dihydroxy-4-pregnen-3-one (DHP), a sexual progestin in teleosts. In the present study, we investigatedin vitrothe brain transcriptional differences between DHP-treated tissues and control groups using RNA-seq. Differential expression analysis revealed that 2700 genes significantly differentially expressed, including 1532 up-regulated and 1168 down-regulated genes. The majority of prostaglandin pathway-related genes were dramatically up-regulated, especially the prostaglandin receptor 6 (ptger6). Tissue distribution analysis revealed thatptger6gene was ubiquitously expressed.In situ hybridizationresults showed thatptger6, nuclear progestin receptor (pgr), and DHP-inducedc-fosmRNA were co-expressed in the ventral telencephalic area, the ventral nucleus of ventral telencephalic area, the anterior part of parvocellular preoptic nucleus, the magnocellular part of magnocellular preoptic nucleus, the ventral zone of periventricular hypothalamus, the anterior tubercular nucleus, the periventricular nucleus of posterior tuberculum, and the torus longitudinalis. DHP significantly enhanced promoter activities ofptger6 viaPgr. Together, this study suggested that DHP regulates the prostaglandin pathway in the neuroendocrine system of teleost fish.