Localization of the mouse Mcf-2 (Dbl) protooncogene within a conserved linkage group on the mouse X chromosome.
Localization of the mouse Mcf-2 (Dbl) protooncogene within a conserved linkage group on the mouse X chromosome.
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小鼠 Mcf-2 (Dbl) 原癌基因在小鼠 X 染色体上保守连锁群内的定位。
DOI：
10.1159/000132988
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Chapman,VM
中科院分区：
文献类型：
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作者：
Grant,SG;Mattei,MG;Galland,F;Stephenson,DA;Keitz,BT;Birnbaum,D;Chapman,VM
与人MCF 2转化序列同源的小鼠cDNA探针已被鉴定和部分克隆，并在此用于定位小鼠X染色体上的基因。人类基因已被物理定位到凝血因子IX基因的60 kb以内，位于小鼠和人类基因组之间的一个大型保守连锁群内，该连锁群在两种哺乳动物物种的X染色体上从HPRT延伸到G6 PD。小鼠Mcf-2探针在小鼠中期染色体上的原位杂交表明，该基因与小鼠凝血因子IX基因Cf-9位于X染色体的同一区域。此外，在来自两个大的种间小鼠回交群体的总共203个个体中，Mcf-2和Cf-9的种特异性基因组DNA多态性的分离（也分离了17个其他X连锁分子标记）表明小鼠基因仅分离0.5 ± 0.5cM。尽管距离很短，我们还是能够将Mcf-2和Cf-9相对于彼此以及该区域的其他基因进行排序。小鼠基因顺序Hprt-Cf-9 - Mcf-2 -G6 pd预测了人类X染色体上基因的类似顺序，这是最近才通过物理作图证明的基因顺序。因此，图的位置和连锁关系的theMcf-2基因是相似的，在人和小鼠，这个独特的原癌基因座是一个保守的连锁群的哺乳动物X染色体上的一部分。
A mouse cDNA probe homologous to the human MCF2 transforming sequence has been identified and partially cloned, and is used here to localize the gene on the mouse X chromosome. The human gene has been physically mapped to within 60 kb of the gene for coagulation factor IX, within a large conserved linkage group between the mouse and human genomes which extends from HPRT to G6PD on the X chromosomes of both mammalian species. In situ hybridization of the mouseMcf-2probe onto mouse metaphase chromosomes indicates that this gene lies in the same region of the X chromosome asCf-9,the mouse gene for coagulation factor IX. Moreover, segregation of species-specific genomic DNA polymorphisms forMcf-2andCf-9in a total of 203 individuals derived from two large interspecific mouse backcross populations (which are also segregating for 17 other X-linked molecular markers) demonstrates that the mouse genes are separated by only 0.5 ± 0.5 cM. Despite this short distance we were able to orderMcf-2andCf-9relative to one another and other genes in this region. The mouse gene orderHprt – Cf-9 – Mcf-2 – G6pdpredicts a similar ordering of genes on the human X chromosome, a gene order which has only recently been demonstrated by physical mapping. Thus, the map location and linkage relationships of theMcf-2gene are similar in man and mouse, and this unique protooncogenic locus is part of a conserved linkage group on the mammalian X chromosome.